Pseudomonas indica -Mediated Silver Nanoparticles: Antifungal and Antioxidant Biogenic Tool for Suppressing Mucormycosis Fungi

• Published in: Journal of fungi (Basel) 2022-01, Vol.8 (2), p.126
• Main Authors: Salem, Salem S, Ali, Omar M, Reyad, Ahmed M, Abd-Elsalam, Kamel A, Hashem, Amr H
• Format: Article
• Language: English
• Subjects: Antifungal activity; antioxidant activity; Antioxidants; Ascorbic acid; Bacteria; Biomass; Biosynthesis; Cancer; Cytotoxicity; Drug resistance; Fourier transforms; Fungi; green biosynthesis; Infrared spectroscopy; Microorganisms; Minimum inhibitory concentration; Mucormycosis; Nanoparticles; Pseudomonas; Science; Silver; silver nanoparticles; Transmission electron microscopy; X-ray diffraction
• ISSN: 2309-608X; 2309-608X
• DOI: 10.3390/jof8020126

Mucormycosis is considered one of the most dangerous invasive fungal diseases. In this study, a facile, green and eco-friendly method was used to biosynthesize silver nanoparticles (AgNPs) using S. Azhar, to combat fungi causing mucormycosis. The biosynthesis of AgNPs was validated by a progressive shift in the color of filtrate from colorless to brown, as well as the identification of a distinctive absorption peak at 420 nm using UV-vis spectroscopy. Fourier-transform infrared spectroscopy (FTIR) results indicated the existence of bioactive chemicals that are responsible for AgNP production. AgNPs with particle sizes ranging from 2.4 to 53.5 nm were discovered using transmission electron microscopy (TEM). Pattern peaks corresponding to the 111, 200, 220, 311, and 222 planes, which corresponded to face-centered cubic forms of metallic silver, were also discovered using X-ray diffraction (XRD). Moreover, antifungal activity measurements of biosynthesized AgNPs against , and were carried out. Results of antifungal activity analysis revealed that the biosynthesized AgNPs exhibited outstanding antifungal activity against all tested fungi at a concentration of 400 µg/mL, where minimum inhibitory concentrations (MIC) were 50, 50, and 100 µg/mL toward , and respectively. In addition, the biosynthesized AgNPs revealed antioxidant activity, where IC was 31 µg/mL when compared to ascorbic acid (0.79 µg/mL). Furthermore, the biosynthesized AgNPs showed no cytotoxicity on the Vero normal cell line. In conclusion, the biosynthesized AgNPs in this study can be used as effective antifungals with safe use, particularly for fungi causing mucormycosis.