Solexa sequencing of novel and differentially expressed microRNAs in testicular and ovarian tissues in Holstein cattle

The posttranscriptional gene regulation mediated by microRNA plays an important role in the development and function of male and female reproductive organs and germ cells in mammals, including cattle. In the present study, we identified novel and differentially expressed miRNAs in the testis and ova...

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Bibliographic Details
Published in:International journal of biological sciences 2011-01, Vol.7 (7), p.1016-1026
Main Authors: Huang, Jinming, Ju, Zhihua, Li, Qiuling, Hou, Qinlei, Wang, Changfa, Li, Jianbin, Li, Rongling, Wang, Lingling, Sun, Tao, Hang, Suqin, Gao, Yundong, Hou, Minghai, Zhong, Jifeng
Format: Article
Language:English
Subjects:
Animals
Cattle - genetics
Computational Biology
Female
Male
MicroRNAs - chemistry
MicroRNAs - genetics
MicroRNAs - metabolism
Ovary - metabolism
Real-Time Polymerase Chain Reaction
Research Paper
Sequence Analysis, RNA
Sex Characteristics
Testis - metabolism
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Summary:The posttranscriptional gene regulation mediated by microRNA plays an important role in the development and function of male and female reproductive organs and germ cells in mammals, including cattle. In the present study, we identified novel and differentially expressed miRNAs in the testis and ovary in Holstein cattle by combining the Solexa sequencing with bioinformatics. In total 100 and 104 novel pre-miRNAs were identified in testicular and ovarian tissues, encoding 122 and 136 mature miRNAs, respectively. Of these, 6 miRNAs appear to be bovine-specific. A total of 246 known miRNAs were co-expressed in the testicular and ovarian tissues. Of the known miRNAs, twenty-one testis-specific and nine ovary-specific (1-23 reads) were found. Approximately 30.5% of the known bovine miRNAs in this study were found to have >2-fold differential expression within the two respective reproductive organ systems. The putative miRNA target genes of miRNAs were involved in pathways associated with reproductive physiology. Both known and novel tissue-specific miRNAs are expressed by Real-time quantitative PCR analysis in dairy cattle. This study expands the number of miRNAs known to be expressed in cattle. The patterns of miRNAs expression differed significantly between the bovine testicular and ovarian tissues, which provide important information on sex differences in miRNA expression. Diverse miRNAs may play an important regulatory role in the development of the reproductive organs in Holstein cattle.
ISSN:1449-2288
1449-2288
DOI:10.7150/ijbs.7.1016