Overexpression of △12, △15-Desaturases for Enhanced Lipids Synthesis in Yarrowia lipolytica

• Published in: Frontiers in microbiology 2020-02, Vol.11, p.289-289
• Main Authors: Yan, Feng Xin, Dong, Gui Ru, Qiang, Shan, Niu, Yong Jie, Hu, Ching Yuan, Meng, Yong Hong
• Format: Article
• Language: English
• Subjects: fatty acid desaturases; lipid production; metabolic engineering; Microbiology; unsaturated fatty acids; Yarrowia lipolytica
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2020.00289

Microbial oil triacylglycerol (TAG) from the renewable feedstock attract much attention. The oleaginous yeast has become the most studied for lipid biosynthesis. Fatty acid desaturases catalyze the introduction of a double bond into fatty-acid hydrocarbon chains to produce unsaturated fatty acids. Desaturases are known to enhance lipid accumulation. In this study, we have achieved a significant increase in lipid production and increase the unsaturated fatty acids content in . By comparing the expression of the native genes of △-9 stearoyl-CoA desaturase (SCD) and △12 desaturase (△12D), and an exogenous △15 desaturase (△15D) from flax in the strain with deleted peroxisomal biogenesis factor 10 (PEX10) and overexpressed diacylglyceride acyl-transferase (DGA1), we found that the strain with overexpressed △15 desaturase accumulated 30.7% lipid. Simultaneously, we explored the effect of two copies of desaturase genes ( - , - , - ) on lipid production, and found co-expression of △ and △ accumulated 42.6% lipid. The lipid content was further increased by 56.3% through the deletion of the multifunctional enzyme (MFE1) and the overexpression of acetyl-CoA carboxylase (ACC1). Finally, the lipid productivity of 50 g/L and maximal lipid content of 77.8% DCW are obtained using a 5-L stirred-tank bioreactor during the stationary phase in the engineered YL-10. Our result demonstrated that the △12 and △15 desaturases play an important role in lipid production in and provides an effective strategy for biodiesel development.