Single-cell transcriptome analysis of medaka lymphocytes reveals absence of fully mature T cells in the thymus and the T-lineage commitment in the kidney

The cellular and molecular mechanisms underlying lymphocyte development are diverse among teleost species. Although recent scRNA-seq analyses of zebrafish hematopoietic cells have advanced our understanding of teleost hematopoiesis, comparative studies using another genetic model, medaka, which is e...

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Bibliographic Details
Published in:Frontiers in immunology 2025-01, Vol.15, p.1517467
Main Authors: Sakaguchi, Hiyori, Matsuda, Masaru, Iwanami, Norimasa
Format: Article
Language:English
Subjects:
Animals
Cell Lineage - genetics
Fish Proteins - genetics
Fish Proteins - immunology
Fish Proteins - metabolism
Gene Expression Profiling
Homeodomain Proteins - genetics
immune system
Immunology
Kidney - cytology
Kidney - immunology
Kidney - metabolism
lymphopoiesis
medaka
Oryzias - genetics
Oryzias - immunology
rag1
scRNA-seq
Single-Cell Analysis
Single-Cell Gene Expression Analysis
T-Lymphocytes - immunology
T-Lymphocytes - metabolism
Thymus Gland - cytology
Thymus Gland - immunology
Thymus Gland - metabolism
Transcriptome
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Summary:The cellular and molecular mechanisms underlying lymphocyte development are diverse among teleost species. Although recent scRNA-seq analyses of zebrafish hematopoietic cells have advanced our understanding of teleost hematopoiesis, comparative studies using another genetic model, medaka, which is evolutionarily distant among teleosts, is useful for understanding commonality and species-specificity in teleosts. In order to gain insight into how different molecular and cellular mechanisms of lymphocyte development in medaka and zebrafish, we established a ( ) mutant medaka, which exhibited defects in V(D)J rearrangement of lymphocyte antigen receptor genes, accordingly lacking mature B and T cells. scRNA-seq analysis of wild type and mutant lymphocytes in the thymus and kidney characterized the developing stages of T and B cells, and found that most developed and single-positive (SP) T-cell populations are absent in the thymus, and identified lymphoid progenitor cells already committed to the T lineage in kidney, implying unique features of medaka lymphocyte development.
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2024.1517467