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Role of Volatiles from the Endophytic Fungus Trichoderma asperelloides PSU-P1 in Biocontrol Potential and in Promoting the Plant Growth of Arabidopsis thaliana
Fungal volatile organic compounds (VOCs) emitted by species interact with a plant host and display multifaceted mechanisms. In this study, we investigated the antifungal activity of VOCs emitted by PSU-P1 against fungal pathogens, as well as the ability of VOCs to activate defense responses and to p...
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| Published in: | Journal of fungi (Basel) 2020-12, Vol.6 (4), p.341 |
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| container_title | Journal of fungi (Basel) |
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| creator | Phoka, Nongnat Suwannarach, Nakarin Lumyong, Saisamorn Ito, Shin-Ichi Matsui, Kenji Arikit, Siwaret Sunpapao, Anurag |
| description | Fungal volatile organic compounds (VOCs) emitted by
species interact with a plant host and display multifaceted mechanisms. In this study, we investigated the antifungal activity of VOCs emitted by
PSU-P1 against fungal pathogens, as well as the ability of VOCs to activate defense responses and to promote plant growth in
. The strain's VOCs had remarkable antifungal activity against fungal pathogens, with an inhibition range of 15.92-84.95% in a volatile antifungal bioassay. The VOCs of
PSU-P1 promoted the plant growth of
, thereby increasing the fresh weight, root length, and chlorophyll content in the VOC-treated
relative to those of the control. High expression levels of the chitinase (
) and β-1,3-glucanase (
) genes were found in the VOC-treated
by quantitative reverse transcription polymerase chain reaction (RT-PCR). The VOC-treated
had higher defense-related enzyme (peroxidase (
)) and cell wall-degrading enzyme (chitinase and β-1,3-glucanase) activity than in the control. The headspace VOCs produced by PSU-P1, trapped with solid phase microextraction, and tentatively identified by gas chromatography-mass spectrometry, included 2-methyl-1-butanol, 2-pentylfuran, acetic acid, and 6-pentyl-2H-pyran-2-one (6-PP). The results suggest that
PSU-P1 emits VOCs responsible for antifungal activity, for promoting plant growth, and for inducing defense responses in
. |
| doi_str_mv | 10.3390/jof6040341 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_8666f8b53a0e4b9dbdaa86296c13e3a4</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_8666f8b53a0e4b9dbdaa86296c13e3a4</doaj_id><sourcerecordid>2468670523</sourcerecordid><originalsourceid>FETCH-LOGICAL-c538t-3bcc18e2566b39b0838b96671fc2da8f137dfa8093a4855ecf087ed46b011f523</originalsourceid><addsrcrecordid>eNpdkl1rFDEYhQdRbKm98QdIwBsRVpPJTCa5EWrpFxRctBXvwjv52M2STdYko_TX-Feb7dbaepWQc_K8h8PbNK8J_kCpwB9X0TLcYdqRZ81-S7GYMcx_PH9032sOc15hjEnPmRD0ZbNHaStIO4j95s_X6A2KFn2PHorzJiOb4hqVpUEnQcfN8qY4hU6nsJgyukpOLaM2aQ0I8sYk4310un6af7uezQlyAX12UcVQUvRoHosJxYFHEPRWm1d0LC4s7vhzD6GgsxR_l-U2wlGC0dWR2eWqg3cQ4FXzwoLP5vD-PGiuT0-ujs9nl1_OLo6PLmeqp7zM6KgU4abtGRupGDGnfBSMDcSqVgO3hA7aAseCQsf73iiL-WB0x0ZMiO1betBc7Lg6wkpukltDupERnLx7iGkhIdUmvJGcMWb52FPAphuFHjUAZ61gilBT-ZX1acfaTOPaaFU7SOCfQJ8qwS3lIv6Sw8BaLIYKeHcPSPHnZHKRa5dV7RqCiVOWbcc4G3CNXa1v_7Ou4pRCrWrrEh0hHG-B73culWLOydiHMATL7RrJf2tUzW8ex3-w_l0aegv3X8VG</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2469411807</pqid></control><display><type>article</type><title>Role of Volatiles from the Endophytic Fungus Trichoderma asperelloides PSU-P1 in Biocontrol Potential and in Promoting the Plant Growth of Arabidopsis thaliana</title><source>Publicly Available Content (ProQuest)</source><source>PubMed Central</source><creator>Phoka, Nongnat ; Suwannarach, Nakarin ; Lumyong, Saisamorn ; Ito, Shin-Ichi ; Matsui, Kenji ; Arikit, Siwaret ; Sunpapao, Anurag</creator><creatorcontrib>Phoka, Nongnat ; Suwannarach, Nakarin ; Lumyong, Saisamorn ; Ito, Shin-Ichi ; Matsui, Kenji ; Arikit, Siwaret ; Sunpapao, Anurag</creatorcontrib><description>Fungal volatile organic compounds (VOCs) emitted by
species interact with a plant host and display multifaceted mechanisms. In this study, we investigated the antifungal activity of VOCs emitted by
PSU-P1 against fungal pathogens, as well as the ability of VOCs to activate defense responses and to promote plant growth in
. The strain's VOCs had remarkable antifungal activity against fungal pathogens, with an inhibition range of 15.92-84.95% in a volatile antifungal bioassay. The VOCs of
PSU-P1 promoted the plant growth of
, thereby increasing the fresh weight, root length, and chlorophyll content in the VOC-treated
relative to those of the control. High expression levels of the chitinase (
) and β-1,3-glucanase (
) genes were found in the VOC-treated
by quantitative reverse transcription polymerase chain reaction (RT-PCR). The VOC-treated
had higher defense-related enzyme (peroxidase (
)) and cell wall-degrading enzyme (chitinase and β-1,3-glucanase) activity than in the control. The headspace VOCs produced by PSU-P1, trapped with solid phase microextraction, and tentatively identified by gas chromatography-mass spectrometry, included 2-methyl-1-butanol, 2-pentylfuran, acetic acid, and 6-pentyl-2H-pyran-2-one (6-PP). The results suggest that
PSU-P1 emits VOCs responsible for antifungal activity, for promoting plant growth, and for inducing defense responses in
.</description><identifier>ISSN: 2309-608X</identifier><identifier>EISSN: 2309-608X</identifier><identifier>DOI: 10.3390/jof6040341</identifier><identifier>PMID: 33291279</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Acetic acid ; Antifungal activity ; Arabidopsis ; Arabidopsis thaliana ; Bioassays ; biocontrol agent ; Biological control ; Butanol ; Cell culture ; Cell walls ; Chitinase ; Chlorophyll ; Endophytes ; Enzymes ; Fungi ; Gas chromatography ; Gene expression ; Headspace ; Host plants ; Mass spectroscopy ; Metabolites ; Microorganisms ; Organic compounds ; Pathogens ; Peroxidase ; Plant diseases ; Plant growth ; Polymerase chain reaction ; Reverse transcription ; Seeds ; Solid phase methods ; Trichoderma ; VOCs ; volatile compounds ; Volatile organic compounds ; Volatiles</subject><ispartof>Journal of fungi (Basel), 2020-12, Vol.6 (4), p.341</ispartof><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c538t-3bcc18e2566b39b0838b96671fc2da8f137dfa8093a4855ecf087ed46b011f523</citedby><cites>FETCH-LOGICAL-c538t-3bcc18e2566b39b0838b96671fc2da8f137dfa8093a4855ecf087ed46b011f523</cites><orcidid>0000-0002-4875-5176 ; 0000-0002-2653-1913 ; 0000-0002-3208-4056 ; 0000-0002-2964-1886</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2469411807/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2469411807?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,74998</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33291279$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Phoka, Nongnat</creatorcontrib><creatorcontrib>Suwannarach, Nakarin</creatorcontrib><creatorcontrib>Lumyong, Saisamorn</creatorcontrib><creatorcontrib>Ito, Shin-Ichi</creatorcontrib><creatorcontrib>Matsui, Kenji</creatorcontrib><creatorcontrib>Arikit, Siwaret</creatorcontrib><creatorcontrib>Sunpapao, Anurag</creatorcontrib><title>Role of Volatiles from the Endophytic Fungus Trichoderma asperelloides PSU-P1 in Biocontrol Potential and in Promoting the Plant Growth of Arabidopsis thaliana</title><title>Journal of fungi (Basel)</title><addtitle>J Fungi (Basel)</addtitle><description>Fungal volatile organic compounds (VOCs) emitted by
species interact with a plant host and display multifaceted mechanisms. In this study, we investigated the antifungal activity of VOCs emitted by
PSU-P1 against fungal pathogens, as well as the ability of VOCs to activate defense responses and to promote plant growth in
. The strain's VOCs had remarkable antifungal activity against fungal pathogens, with an inhibition range of 15.92-84.95% in a volatile antifungal bioassay. The VOCs of
PSU-P1 promoted the plant growth of
, thereby increasing the fresh weight, root length, and chlorophyll content in the VOC-treated
relative to those of the control. High expression levels of the chitinase (
) and β-1,3-glucanase (
) genes were found in the VOC-treated
by quantitative reverse transcription polymerase chain reaction (RT-PCR). The VOC-treated
had higher defense-related enzyme (peroxidase (
)) and cell wall-degrading enzyme (chitinase and β-1,3-glucanase) activity than in the control. The headspace VOCs produced by PSU-P1, trapped with solid phase microextraction, and tentatively identified by gas chromatography-mass spectrometry, included 2-methyl-1-butanol, 2-pentylfuran, acetic acid, and 6-pentyl-2H-pyran-2-one (6-PP). The results suggest that
PSU-P1 emits VOCs responsible for antifungal activity, for promoting plant growth, and for inducing defense responses in
.</description><subject>Acetic acid</subject><subject>Antifungal activity</subject><subject>Arabidopsis</subject><subject>Arabidopsis thaliana</subject><subject>Bioassays</subject><subject>biocontrol agent</subject><subject>Biological control</subject><subject>Butanol</subject><subject>Cell culture</subject><subject>Cell walls</subject><subject>Chitinase</subject><subject>Chlorophyll</subject><subject>Endophytes</subject><subject>Enzymes</subject><subject>Fungi</subject><subject>Gas chromatography</subject><subject>Gene expression</subject><subject>Headspace</subject><subject>Host plants</subject><subject>Mass spectroscopy</subject><subject>Metabolites</subject><subject>Microorganisms</subject><subject>Organic compounds</subject><subject>Pathogens</subject><subject>Peroxidase</subject><subject>Plant diseases</subject><subject>Plant growth</subject><subject>Polymerase chain reaction</subject><subject>Reverse transcription</subject><subject>Seeds</subject><subject>Solid phase methods</subject><subject>Trichoderma</subject><subject>VOCs</subject><subject>volatile compounds</subject><subject>Volatile organic compounds</subject><subject>Volatiles</subject><issn>2309-608X</issn><issn>2309-608X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdkl1rFDEYhQdRbKm98QdIwBsRVpPJTCa5EWrpFxRctBXvwjv52M2STdYko_TX-Feb7dbaepWQc_K8h8PbNK8J_kCpwB9X0TLcYdqRZ81-S7GYMcx_PH9032sOc15hjEnPmRD0ZbNHaStIO4j95s_X6A2KFn2PHorzJiOb4hqVpUEnQcfN8qY4hU6nsJgyukpOLaM2aQ0I8sYk4310un6af7uezQlyAX12UcVQUvRoHosJxYFHEPRWm1d0LC4s7vhzD6GgsxR_l-U2wlGC0dWR2eWqg3cQ4FXzwoLP5vD-PGiuT0-ujs9nl1_OLo6PLmeqp7zM6KgU4abtGRupGDGnfBSMDcSqVgO3hA7aAseCQsf73iiL-WB0x0ZMiO1betBc7Lg6wkpukltDupERnLx7iGkhIdUmvJGcMWb52FPAphuFHjUAZ61gilBT-ZX1acfaTOPaaFU7SOCfQJ8qwS3lIv6Sw8BaLIYKeHcPSPHnZHKRa5dV7RqCiVOWbcc4G3CNXa1v_7Ou4pRCrWrrEh0hHG-B73culWLOydiHMATL7RrJf2tUzW8ex3-w_l0aegv3X8VG</recordid><startdate>20201206</startdate><enddate>20201206</enddate><creator>Phoka, Nongnat</creator><creator>Suwannarach, Nakarin</creator><creator>Lumyong, Saisamorn</creator><creator>Ito, Shin-Ichi</creator><creator>Matsui, Kenji</creator><creator>Arikit, Siwaret</creator><creator>Sunpapao, Anurag</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-4875-5176</orcidid><orcidid>https://orcid.org/0000-0002-2653-1913</orcidid><orcidid>https://orcid.org/0000-0002-3208-4056</orcidid><orcidid>https://orcid.org/0000-0002-2964-1886</orcidid></search><sort><creationdate>20201206</creationdate><title>Role of Volatiles from the Endophytic Fungus Trichoderma asperelloides PSU-P1 in Biocontrol Potential and in Promoting the Plant Growth of Arabidopsis thaliana</title><author>Phoka, Nongnat ; Suwannarach, Nakarin ; Lumyong, Saisamorn ; Ito, Shin-Ichi ; Matsui, Kenji ; Arikit, Siwaret ; Sunpapao, Anurag</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c538t-3bcc18e2566b39b0838b96671fc2da8f137dfa8093a4855ecf087ed46b011f523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acetic acid</topic><topic>Antifungal activity</topic><topic>Arabidopsis</topic><topic>Arabidopsis thaliana</topic><topic>Bioassays</topic><topic>biocontrol agent</topic><topic>Biological control</topic><topic>Butanol</topic><topic>Cell culture</topic><topic>Cell walls</topic><topic>Chitinase</topic><topic>Chlorophyll</topic><topic>Endophytes</topic><topic>Enzymes</topic><topic>Fungi</topic><topic>Gas chromatography</topic><topic>Gene expression</topic><topic>Headspace</topic><topic>Host plants</topic><topic>Mass spectroscopy</topic><topic>Metabolites</topic><topic>Microorganisms</topic><topic>Organic compounds</topic><topic>Pathogens</topic><topic>Peroxidase</topic><topic>Plant diseases</topic><topic>Plant growth</topic><topic>Polymerase chain reaction</topic><topic>Reverse transcription</topic><topic>Seeds</topic><topic>Solid phase methods</topic><topic>Trichoderma</topic><topic>VOCs</topic><topic>volatile compounds</topic><topic>Volatile organic compounds</topic><topic>Volatiles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Phoka, Nongnat</creatorcontrib><creatorcontrib>Suwannarach, Nakarin</creatorcontrib><creatorcontrib>Lumyong, Saisamorn</creatorcontrib><creatorcontrib>Ito, Shin-Ichi</creatorcontrib><creatorcontrib>Matsui, Kenji</creatorcontrib><creatorcontrib>Arikit, Siwaret</creatorcontrib><creatorcontrib>Sunpapao, Anurag</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>Biological Sciences</collection><collection>Biological Science Database</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Journal of fungi (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Phoka, Nongnat</au><au>Suwannarach, Nakarin</au><au>Lumyong, Saisamorn</au><au>Ito, Shin-Ichi</au><au>Matsui, Kenji</au><au>Arikit, Siwaret</au><au>Sunpapao, Anurag</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of Volatiles from the Endophytic Fungus Trichoderma asperelloides PSU-P1 in Biocontrol Potential and in Promoting the Plant Growth of Arabidopsis thaliana</atitle><jtitle>Journal of fungi (Basel)</jtitle><addtitle>J Fungi (Basel)</addtitle><date>2020-12-06</date><risdate>2020</risdate><volume>6</volume><issue>4</issue><spage>341</spage><pages>341-</pages><issn>2309-608X</issn><eissn>2309-608X</eissn><abstract>Fungal volatile organic compounds (VOCs) emitted by
species interact with a plant host and display multifaceted mechanisms. In this study, we investigated the antifungal activity of VOCs emitted by
PSU-P1 against fungal pathogens, as well as the ability of VOCs to activate defense responses and to promote plant growth in
. The strain's VOCs had remarkable antifungal activity against fungal pathogens, with an inhibition range of 15.92-84.95% in a volatile antifungal bioassay. The VOCs of
PSU-P1 promoted the plant growth of
, thereby increasing the fresh weight, root length, and chlorophyll content in the VOC-treated
relative to those of the control. High expression levels of the chitinase (
) and β-1,3-glucanase (
) genes were found in the VOC-treated
by quantitative reverse transcription polymerase chain reaction (RT-PCR). The VOC-treated
had higher defense-related enzyme (peroxidase (
)) and cell wall-degrading enzyme (chitinase and β-1,3-glucanase) activity than in the control. The headspace VOCs produced by PSU-P1, trapped with solid phase microextraction, and tentatively identified by gas chromatography-mass spectrometry, included 2-methyl-1-butanol, 2-pentylfuran, acetic acid, and 6-pentyl-2H-pyran-2-one (6-PP). The results suggest that
PSU-P1 emits VOCs responsible for antifungal activity, for promoting plant growth, and for inducing defense responses in
.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33291279</pmid><doi>10.3390/jof6040341</doi><orcidid>https://orcid.org/0000-0002-4875-5176</orcidid><orcidid>https://orcid.org/0000-0002-2653-1913</orcidid><orcidid>https://orcid.org/0000-0002-3208-4056</orcidid><orcidid>https://orcid.org/0000-0002-2964-1886</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of fungi (Basel), 2020-12, Vol.6 (4), p.341 |
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| source | Publicly Available Content (ProQuest); PubMed Central |
| subjects | Acetic acid Antifungal activity Arabidopsis Arabidopsis thaliana Bioassays biocontrol agent Biological control Butanol Cell culture Cell walls Chitinase Chlorophyll Endophytes Enzymes Fungi Gas chromatography Gene expression Headspace Host plants Mass spectroscopy Metabolites Microorganisms Organic compounds Pathogens Peroxidase Plant diseases Plant growth Polymerase chain reaction Reverse transcription Seeds Solid phase methods Trichoderma VOCs volatile compounds Volatile organic compounds Volatiles |
| title | Role of Volatiles from the Endophytic Fungus Trichoderma asperelloides PSU-P1 in Biocontrol Potential and in Promoting the Plant Growth of Arabidopsis thaliana |
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