Ethanol- and PARP-Mediated Regulation of Ribosome-Associated Long Non-Coding RNA (lncRNA) in Pyramidal Neurons

Although, by definition, long noncoding RNAs (lncRNAs) are not translated, they are sometimes associated with ribosomes. In fact, some estimates suggest the existence of more than 50 K lncRNA molecules that could encode for small peptides. We examined the effects of an ethanol and Poly-ADP Ribose Po...

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Bibliographic Details
Published in:Non-coding RNA 2023-11, Vol.9 (6), p.72
Main Authors: Rizavi, Hooriyah S, Gavin, Hannah E, Krishnan, Harish R, Gavin, David P, Sharma, Rajiv P
Format: Article
Language:English
Subjects:
ABT-888
Acetaldehyde
Alcohol
alcohol use disorder
Alcohol, Denatured
Analysis
Cell cycle
Cytotoxicity
Ethanol
Gene expression
Lymphocytes T
MicroRNAs
Monosaccharides
Neurons
Neurophysiology
Non-coding RNA
PARP inhibitor
Poly(ADP-ribose) polymerase
prefrontal cortex
Proteins
Pyramidal cells
Ribonucleic acid
ribosome-bound lncRNA
Ribosomes
RNA
RNA polymerase
Stem cells
Sugars
T cells
TRAP
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Summary:Although, by definition, long noncoding RNAs (lncRNAs) are not translated, they are sometimes associated with ribosomes. In fact, some estimates suggest the existence of more than 50 K lncRNA molecules that could encode for small peptides. We examined the effects of an ethanol and Poly-ADP Ribose Polymerase (PARP) inhibitor (ABT-888) on ribosome-bound lncRNAs. Mice were administered via intraperitoneal injection (i.p.) either normal saline (CTL) or ethanol (EtOH) twice a day for four consecutive days. On the fourth day, a sub-group of mice administered with ethanol also received ABT-888 (EtOH+ABT). Ribosome-bound lncRNAs in CaMKIIα-expressing pyramidal neurons were measured using the Translating Ribosome Affinity Purification (TRAP) technique. Our findings show that EtOH altered the attachment of 107 lncRNA transcripts, while EtOH+ABT altered 60 lncRNAs. Among these 60 lncRNAs, 49 were altered by both conditions, while EtOH+ABT uniquely altered the attachment of 11 lncRNA transcripts that EtOH alone did not affect. To validate these results, we selected eight lncRNAs (Mir124-2hg, 5430416N02Rik, Snhg17, Snhg12, Snhg1, Mir9-3hg, Gas5, and 1110038B12Rik) for qRT-PCR analysis. The current study demonstrates that ethanol-induced changes in lncRNA attachment to ribosomes can be mitigated by the addition of the PARP inhibitor ABT-888.
ISSN:2311-553X
2311-553X
DOI:10.3390/ncrna9060072