Association of Plasma Irisin Levels with Circulating Endothelial Microparticles (EMPs) and Endothelial Progenitor Cells (EPCs) in Children Born Prematurely

• Published in: Metabolites 2023-01, Vol.13 (1), p.120
• Main Authors: Markopoulou, Panagiota, Koutroumpa, Arsinoi, Mantzou, Aimilia, Margeli, Alexandra, Papanikolaou, Eleni, Siahanidou, Tania
• Format: Article
• Language: English
• Subjects: Birth weight; Blood pressure; Body fat; Body mass index; Body measurements; Bone marrow; Cardiovascular disease; CD34 antigen; CD45 antigen; Children; Diabetes; endothelial dysfunction; endothelial microparticles; endothelial progenitor cells; Endothelium; Exercise; Families & family life; Family medical history; Flow cytometry; Gestational age; Insulin; Investigations; irisin; Lipids; Microparticles; Physical fitness; Population studies; Premature birth; prematurity; Progenitor cells; Software; Statistical power; Stem cells; Vascular endothelial growth factor receptors
• ISSN: 2218-1989; 2218-1989
• DOI: 10.3390/metabo13010120

Prematurity has been linked with endothelial dysfunction in later life. The purpose of this study was to evaluate the association between plasma irisin, an adipomyokine reported to protect the functional integrity of vascular endothelium, and circulating endothelial microparticles (EMPs) and endothelial progenitor cells (EPCs), consisting early biomarkers of endothelial dysfunction, in preterm-born children. We studied 131 prepubertal children; 61 preterm and 70 born at term (controls). Plasma irisin was determined by ELISA. Circulating CD62E(+), CD144(+) and CD31(+)/CD42b(-) EMPs, and CD34(+)/VEGFR-2(+)/CD45(-) and CD34(+)/VEGFR-2(+)/CD45dim EPCs, were determined by flow cytometry. Body mass index, waist-to-hip ratio, neck circumference, systolic and diastolic blood pressure, and biochemical parameters (glucose, lipids, insulin, HOMA-IR) were also evaluated. Plasma irisin was significantly lower ( = 0.001), whereas circulating EMPs and EPCs were higher, in children born prematurely compared to controls. Irisin was recognized as independent predictor for CD144(+) and CD31(+)/CD42b(-) EMPs, CD34(+)/VEGFR-2(+)/CD45(-) and CD34(+)/VEGFR-2(+)/CD45dim EPCs in the total study population, and for CD31(+)/CD42b(-) EMPs in the preterm group. In conclusion, plasma irisin correlates independently with circulating EMP and EPC subpopulations in prepubertal children and in preterm-born ones. Further studies in children will potentially elucidate the link between irisin and the primary stages of prematurity-related endothelial dysfunction.