Snapshot situation of oxidative degradation of the nervous system, kidney, and adrenal glands biomarkers-neuroprostane and dihomo-isoprostanes-urinary biomarkers from infancy to elderly adults

• Published in: Redox biology 2017-04, Vol.11, p.586-591
• Main Authors: García-Flores, Libia Alejandra, Medina, Sonia, Martínez-Hernández, Pedro, Oger, Camille, Galano, Jean-Marie, Durand, Thierry, Casas-Pina, Teresa, Ferreres, Federico, Gil-Izquierdo, Ángel
• Format: Article
• Language: English
• Subjects: Adolescent; Adrenal Glands - metabolism; Adult; Age; Aged; Aged, 80 and over; Analytical chemistry; Biochemistry; Biochemistry, Molecular Biology; Biomarkers - metabolism; Chemical Sciences; Child; Child, Preschool; Dihomo-isoprostanes; F2-Isoprostanes - metabolism; Female; Humans; Kidney - metabolism; Life Sciences; Lipid Peroxidation; Male; Middle Aged; Nervous System - metabolism; Neuroprostanes; Neuroprostanes - metabolism; Oxidation-Reduction; Oxidative Stress; Research Paper; Tandem Mass Spectrometry; Urine biomarkers; Young Adult
• ISSN: 2213-2317; 2213-2317
• DOI: 10.1016/j.redox.2017.01.008

We analyzed biomarkers of lipid peroxidation of the nervous system -F2-dihomo-isoprostanes, F3-neuroprostanes, and F4-neuroprostanes- in urine samples from 158 healthy volunteers ranging from 4 to 88 years old with the aim of analyzing possible associations between their excretion values and age (years). Ten biomarkers were screened in the urine samples by UHPLC-QqQ-MS/MS. Four F2-dihomo-isoprostanes (ent−7-(R)−7-F2t-dihomo-isoprostane, ent−7-epi−7-F2t-dihomo-isoprostane, 17-F2t-dihomo-isoprostane, 17-epi−17-F2t-dihomo-isoprostane), and one DPA-neuroprostane (4-F3t-neuroprostane) were detected in the samples. On the one hand, we found a significant, positive correlation (Rho: 0.197, P=0.015) between the age increase and the amount of total F2-dihomo-IsoPs. On the other hand, the values were significantly higher in the childhood group (4–12 years old), when compared to the adolescence group (13–17 years old) and the young adult group (18–35 years old). Surprisingly, no significant differences were found between the middle-aged adults (36–64 years old) and the elderly adults (65–88 years old). We display a snapshot situation of excretory values of oxidative stress biomarkers of the nervous system, using healthy volunteers representative of the different stages of human growth and development. The values reported in this study could be used as a basal or starting point in clinical interventions related to aging processes and/or pathologies associated with the nervous system. [Display omitted] •Positive correlation between the age and values of F2-dihomo-isoprostanes was found.•F2-dihomo-isoprostanes were significantly higher in childhood group when compared to adolescents and young adults.•F2-dihomo-isoprostanes were not statistically different in middle-aged group and elderly group.•Neuroprostanes were no detected in healthy volunteers.