A Saprophytic Fungus Tubeufia rubra Produces Novel Rubracin D and E Reversing Multidrug Resistance in Cancer Cells

Two new ( , viz Rubracin D and E) and sixteen known Glyceroglycolipids ( - ) in the saprophytic fungus (PF02-2) from decaying wood in freshwater habitat were isolated and identified. Their chemical structures were elucidated via means of the extensive spectroscopic analyses of NMR, HR-ESI-MS and UV...

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Published in:Journal of fungi (Basel) 2023-02, Vol.9 (3), p.309
Main Authors: Qian, Shengyan, Zeng, Xuebo, Qian, Yixin, Lu, Yongzhong, He, Zhangjiang, Kang, Jichuan
Format: Article
Language:English
Subjects:
Cancer
cancer multidrug resistance
Chromatography
Fermentation
Freshwater environments
Fungi
Glyceroglycolipids
Metabolites
Multidrug resistance
Natural products
NMR
Nuclear magnetic resonance
P-Glycoprotein
reverse
secondary metabolites
Tubeufia rubra
Tumor cell lines
Verapamil
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Summary:Two new ( , viz Rubracin D and E) and sixteen known Glyceroglycolipids ( - ) in the saprophytic fungus (PF02-2) from decaying wood in freshwater habitat were isolated and identified. Their chemical structures were elucidated via means of the extensive spectroscopic analyses of NMR, HR-ESI-MS and UV spectra, as well as comparison with literature data. The new compounds were assayed for the reversal activity of multidrug resistance (MDR) on MCF-7/ADM, K562/ADM and A549/ADM cell lines, and both compounds and reversed MDR in the three resistant cancer cell lines with concentration dependence. In the assay on K562/ADM, both new compounds had been proved to have remarkable MDR reversal effects, which were higher than those of the positive control viz Verapamil (Vrp). Meanwhile, in the assay on A549/ADM, compound displayed significant MDR reversal effects, which were also higher than those of Vrp at certain concentrations. Furthermore, the Western blot assay proved that both new compounds reversed the MDR in the resistant cancer cell line viz MCF-7/ADM by inhibiting the overexpression of P-glycoprotein. This is the first report that the Glyceroglycolipids isolated firstly from the fungal genus reversed MDR in resistant cancer cells.
ISSN:2309-608X
2309-608X
DOI:10.3390/jof9030309