Biogenic Amine Levels Markedly Increase in the Aqueous Humor of Individuals with Controlled Type 2 Diabetes

• Published in: International journal of molecular sciences 2022-10, Vol.23 (21), p.12752
• Main Authors: Lillo, Alejandro, Marin, Silvia, Serrano-Marín, Joan, Bernal-Casas, David, Binetti, Nicolas, Navarro, Gemma, Cascante, Marta, Sánchez-Navés, Juan, Franco, Rafael
• Format: Article
• Language: English
• Subjects: Acetic acid; Alanine; Amino acids; Aqueous Humor; Aqueous humour; Biogenic Amines; Comorbidity; Composition; Creatinine; Diabetes; Diabetes mellitus (non-insulin dependent); Diabetes Mellitus, Type 2; Disease; Glaucoma; glutamine; Humans; Lactic acid; Metabolism; Metabolites; Metabolomics; ornithine; Pathology; Patients; Principal components analysis; Pyruvates; Pyruvic acid; type 2 diabetes
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms232112752

The composition of the aqueous humor of patients with type 2 diabetes is relevant to understanding the underlying causes of eye-related comorbidities. Information on the composition of aqueous humor in healthy subjects is limited due to the lack of adequate controls. To carry out a metabolomics study, 31 samples of aqueous humor from healthy subjects without ocular pathology, submitted to refractive surgery and seven samples from patients with type 2 diabetes without signs of ocular pathology related to diabetes were used. The level of 25 molecules was significantly (p < 0.001) altered in the aqueous humor of the patient group. The concentration of a single molecule, N-acetylornithine, makes it possible to discriminate between control and diabetes (sensitivity and specificity equal to 1). In addition, receptor operating characteristic curve and principal component analysis for the above-mentioned six molecules yielded significantly (p < 0.001) altered in the aqueous humor of the patient group. In addition, receptor operating characteristic curve and principal component analysis for six compounds yielded cut-off values and remarkable sensitivity, specificity, and segregation ability. The altered level of N-acetylornithine may be due to an increased amount of acetate in diabetes. It is of interest to further investigate whether this alteration is related to the pathogenesis of the disease. The increase in the amino form of pyruvate, alanine, in diabetes is also relevant because it could be a means of reducing the formation of lactate from pyruvate.