Bifidobacterium longum LBUX23 Isolated from Feces of a Newborn; Potential Probiotic Properties and Genomic Characterization

• Published in: Microorganisms (Basel) 2023-06, Vol.11 (7), p.1648
• Main Authors: Reyes-Castillo, Pedro A, González-Vázquez, Raquel, Torres-Maravilla, Edgar, Bautista-Hernández, Jessica I, Zúñiga-León, Eduardo, Leyte-Lugo, Martha, Mateos-Sánchez, Leovigildo, Mendoza-Pérez, Felipe, Gutiérrez-Nava, María Angélica, Reyes-Pavón, Diana, Azaola-Espinosa, Alejandro, Mayorga-Reyes, Lino
• Format: Article
• Language: English
• Subjects: Alzheimer's disease; Ampicillin; Antibiotics; antioxidant; Antioxidants; Bifidobacterium; Bifidobacterium longum; Bile; Bile salts; BSH activity; Cholesterol; CRISPR; Dicloxacillin; E coli; Enzymes; Feces; Fructooligosaccharides; functional characterization; Genes; Genetic aspects; genome; Genomics; Hydroxyl radicals; Identification and classification; In vivo methods and tests; Lactose; Lactulose; Neonates; Pathogens; Physiological aspects; Plasmids; Probiotics; Scavenging; Sucrose; Superoxide anions; Survival; Taurocholic acid; Taurodeoxycholic acid; Testing; TmRNA
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms11071648

is considered a microorganism with probiotic potential, which has been extensively studied, but these probiotic effects are strain dependent. This work aims to characterize the probiotic potential, based on the biochemical and genomic functionality, of LBUX23, isolated from neonates' feces. LBUX23 contains one circular genome of 2,287,838 bp with a G+C content of 60.05%, no plasmids, no CRISPR-Cas operon, possesses 56 tRNAs, 9 rRNAs, 1 tmRNA and 1776 coding sequences (CDSs). It has chromosomally encoded resistance genes to ampicillin and dicloxacillin, non-hemolytic activity, and moderate inhibition of ATCC 25922 and to some emergent pathogen's clinical strains. LBUX23 was able to utilize lactose, sucrose, fructooligosaccharides (FOS), and lactulose. The maximum peak of bacterial growth was observed in sucrose and FOS at 6 h; in lactose and lactulose, it was shown at 8 h. LBUX23 can survive in gastrointestinal conditions (pH 4 to 7). A decrease in survival (96.5 and 93.8%) was observed at pH 3 and 3.5 during 120 min. , , and genes could be involved in this tolerance. LBUX23 can also survive under primary and secondary glyco- or tauro-conjugated bile salts, and a mixture of bile salts due to the high extracellular bile salt hydrolase (BSH) activity (67.3 %), in taurocholic acid followed by taurodeoxycholic acid (48.5%), glycocholic acid (47.1%), oxgall (44.3%), and glycodeoxycholic acid (29.7%) probably due to the presence of the and genes which form an operon (start: 119573 and end: 123812). Low BSH activity was determined intracellularly (