Loading…
Bifidobacterium longum LBUX23 Isolated from Feces of a Newborn; Potential Probiotic Properties and Genomic Characterization
is considered a microorganism with probiotic potential, which has been extensively studied, but these probiotic effects are strain dependent. This work aims to characterize the probiotic potential, based on the biochemical and genomic functionality, of LBUX23, isolated from neonates' feces. LBU...
Saved in:
| Published in: | Microorganisms (Basel) 2023-06, Vol.11 (7), p.1648 |
|---|---|
| Main Authors: | , , , , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c576t-553cf7b1e98ecedd6e0b27830bfbe27d0377ef4122f3ccf6d43c38da0ca739f93 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c576t-553cf7b1e98ecedd6e0b27830bfbe27d0377ef4122f3ccf6d43c38da0ca739f93 |
| container_end_page | |
| container_issue | 7 |
| container_start_page | 1648 |
| container_title | Microorganisms (Basel) |
| container_volume | 11 |
| creator | Reyes-Castillo, Pedro A González-Vázquez, Raquel Torres-Maravilla, Edgar Bautista-Hernández, Jessica I Zúñiga-León, Eduardo Leyte-Lugo, Martha Mateos-Sánchez, Leovigildo Mendoza-Pérez, Felipe Gutiérrez-Nava, María Angélica Reyes-Pavón, Diana Azaola-Espinosa, Alejandro Mayorga-Reyes, Lino |
| description | is considered a microorganism with probiotic potential, which has been extensively studied, but these probiotic effects are strain dependent. This work aims to characterize the probiotic potential, based on the biochemical and genomic functionality, of
LBUX23, isolated from neonates' feces.
LBUX23 contains one circular genome of 2,287,838 bp with a G+C content of 60.05%, no plasmids, no CRISPR-Cas operon, possesses 56 tRNAs, 9 rRNAs, 1 tmRNA and 1776 coding sequences (CDSs). It has chromosomally encoded resistance genes to ampicillin and dicloxacillin, non-hemolytic activity, and moderate inhibition of
ATCC 25922 and to some emergent pathogen's clinical strains.
LBUX23 was able to utilize lactose, sucrose, fructooligosaccharides (FOS), and lactulose. The maximum peak of bacterial growth was observed in sucrose and FOS at 6 h; in lactose and lactulose, it was shown at 8 h.
LBUX23 can survive in gastrointestinal conditions (pH 4 to 7). A decrease in survival (96.5 and 93.8%) was observed at pH 3 and 3.5 during 120 min.
,
, and
genes could be involved in this tolerance.
LBUX23 can also survive under primary and secondary glyco- or tauro-conjugated bile salts, and a mixture of bile salts due to the high extracellular bile salt hydrolase (BSH) activity (67.3 %), in taurocholic acid followed by taurodeoxycholic acid (48.5%), glycocholic acid (47.1%), oxgall (44.3%), and glycodeoxycholic acid (29.7%) probably due to the presence of the
and
genes which form an operon (start: 119573 and end: 123812). Low BSH activity was determined intracellularly ( |
| doi_str_mv | 10.3390/microorganisms11071648 |
| format | article |
| fullrecord | <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_86e1963ed96040c29801ab44b88e0c06</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A759156841</galeid><doaj_id>oai_doaj_org_article_86e1963ed96040c29801ab44b88e0c06</doaj_id><sourcerecordid>A759156841</sourcerecordid><originalsourceid>FETCH-LOGICAL-c576t-553cf7b1e98ecedd6e0b27830bfbe27d0377ef4122f3ccf6d43c38da0ca739f93</originalsourceid><addsrcrecordid>eNptUk1vEzEQXSEQrUr_QmWJC5cUf-zaXnFAbURLpAh6oBI3y2uPU0e7drA3RdA_Xy8ppUG1DzMav_fmw1NVJwSfMtbi94M3Kca00sHnIROCBeG1fFEdUiz4jHIsXj7xD6rjnNe4nJYw2ZDX1QETDaGSksPq7tw7b2OnzQjJbwfUx7AqZnl-_Z0ytMix1yNY5FIc0AUYyCg6pNEX-NnFFD6gqzhCGL3u0VWKnY-jN5O3gTT6AtbBoksIsZSM5jc67fL81qOP4U31yuk-w_GDPaquLz59m3-eLb9eLuZny5lpBB9nTcOMEx2BVpb81nLAHRWS4c51QIXFTAhwNaHUMWMctzUzTFqNjRasdS07qhY7XRv1Wm2SH3T6paL26k-gDFLpUq3pQUkOpOUMbMtxjQ1tJSa6q-tOSsAG86L1cae12XYDWFN6T7rfE91_Cf5GreKtIniavWRF4d2DQoo_tpBHNfhsoO91gLjNisq6xgXaTsne_gddx20KZVYTimHJuGz-oVa6dOCDiyWxmUTVmWha0nBZk4I6fQZVroXyNzGA8yW-R-A7Qtm1nBO4xyYJVtMaqufXsBBPno7okfZ36dg9IJHdJw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2843083685</pqid></control><display><type>article</type><title>Bifidobacterium longum LBUX23 Isolated from Feces of a Newborn; Potential Probiotic Properties and Genomic Characterization</title><source>Publicly Available Content (ProQuest)</source><source>PubMed Central</source><creator>Reyes-Castillo, Pedro A ; González-Vázquez, Raquel ; Torres-Maravilla, Edgar ; Bautista-Hernández, Jessica I ; Zúñiga-León, Eduardo ; Leyte-Lugo, Martha ; Mateos-Sánchez, Leovigildo ; Mendoza-Pérez, Felipe ; Gutiérrez-Nava, María Angélica ; Reyes-Pavón, Diana ; Azaola-Espinosa, Alejandro ; Mayorga-Reyes, Lino</creator><creatorcontrib>Reyes-Castillo, Pedro A ; González-Vázquez, Raquel ; Torres-Maravilla, Edgar ; Bautista-Hernández, Jessica I ; Zúñiga-León, Eduardo ; Leyte-Lugo, Martha ; Mateos-Sánchez, Leovigildo ; Mendoza-Pérez, Felipe ; Gutiérrez-Nava, María Angélica ; Reyes-Pavón, Diana ; Azaola-Espinosa, Alejandro ; Mayorga-Reyes, Lino</creatorcontrib><description>is considered a microorganism with probiotic potential, which has been extensively studied, but these probiotic effects are strain dependent. This work aims to characterize the probiotic potential, based on the biochemical and genomic functionality, of
LBUX23, isolated from neonates' feces.
LBUX23 contains one circular genome of 2,287,838 bp with a G+C content of 60.05%, no plasmids, no CRISPR-Cas operon, possesses 56 tRNAs, 9 rRNAs, 1 tmRNA and 1776 coding sequences (CDSs). It has chromosomally encoded resistance genes to ampicillin and dicloxacillin, non-hemolytic activity, and moderate inhibition of
ATCC 25922 and to some emergent pathogen's clinical strains.
LBUX23 was able to utilize lactose, sucrose, fructooligosaccharides (FOS), and lactulose. The maximum peak of bacterial growth was observed in sucrose and FOS at 6 h; in lactose and lactulose, it was shown at 8 h.
LBUX23 can survive in gastrointestinal conditions (pH 4 to 7). A decrease in survival (96.5 and 93.8%) was observed at pH 3 and 3.5 during 120 min.
,
, and
genes could be involved in this tolerance.
LBUX23 can also survive under primary and secondary glyco- or tauro-conjugated bile salts, and a mixture of bile salts due to the high extracellular bile salt hydrolase (BSH) activity (67.3 %), in taurocholic acid followed by taurodeoxycholic acid (48.5%), glycocholic acid (47.1%), oxgall (44.3%), and glycodeoxycholic acid (29.7%) probably due to the presence of the
and
genes which form an operon (start: 119573 and end: 123812). Low BSH activity was determined intracellularly (<7%), particularly in glycocholic acid; no intracellular activity was shown.
LBUX23 showed antioxidant effects in DPPH radical, mainly in intact cells (27.4%). In the case of hydroxyl radical scavenging capacity, cell debris showed the highest reduction (72.5%). In the cell-free extract, superoxide anion radical scavenging capacity was higher (90.5%). The genome of
LBUX23 contains
,
,
,
and
genes, which could be involved in this activity. Regarding adherence, it showed adherence up to 5% to Caco-2 cells.
LBUX23 showed in vitro potential probiotic properties, mainly in BSH activity and antioxidant capacity, which indicates that it could be a good candidate for antioxidant or anti-cholesterol tests using in vivo models.</description><identifier>ISSN: 2076-2607</identifier><identifier>EISSN: 2076-2607</identifier><identifier>DOI: 10.3390/microorganisms11071648</identifier><identifier>PMID: 37512821</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Alzheimer's disease ; Ampicillin ; Antibiotics ; antioxidant ; Antioxidants ; Bifidobacterium ; Bifidobacterium longum ; Bile ; Bile salts ; BSH activity ; Cholesterol ; CRISPR ; Dicloxacillin ; E coli ; Enzymes ; Feces ; Fructooligosaccharides ; functional characterization ; Genes ; Genetic aspects ; genome ; Genomics ; Hydroxyl radicals ; Identification and classification ; In vivo methods and tests ; Lactose ; Lactulose ; Neonates ; Pathogens ; Physiological aspects ; Plasmids ; Probiotics ; Scavenging ; Sucrose ; Superoxide anions ; Survival ; Taurocholic acid ; Taurodeoxycholic acid ; Testing ; TmRNA</subject><ispartof>Microorganisms (Basel), 2023-06, Vol.11 (7), p.1648</ispartof><rights>COPYRIGHT 2023 MDPI AG</rights><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 by the authors. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c576t-553cf7b1e98ecedd6e0b27830bfbe27d0377ef4122f3ccf6d43c38da0ca739f93</citedby><cites>FETCH-LOGICAL-c576t-553cf7b1e98ecedd6e0b27830bfbe27d0377ef4122f3ccf6d43c38da0ca739f93</cites><orcidid>0000-0002-3359-4517 ; 0000-0002-3850-1244 ; 0000-0003-4940-6194 ; 0000-0003-4400-7506 ; 0000-0001-8051-9432 ; 0000-0001-9768-2842 ; 0000-0001-6342-5549</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2843083685/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2843083685?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25752,27923,27924,37011,37012,44589,53790,53792,74897</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37512821$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reyes-Castillo, Pedro A</creatorcontrib><creatorcontrib>González-Vázquez, Raquel</creatorcontrib><creatorcontrib>Torres-Maravilla, Edgar</creatorcontrib><creatorcontrib>Bautista-Hernández, Jessica I</creatorcontrib><creatorcontrib>Zúñiga-León, Eduardo</creatorcontrib><creatorcontrib>Leyte-Lugo, Martha</creatorcontrib><creatorcontrib>Mateos-Sánchez, Leovigildo</creatorcontrib><creatorcontrib>Mendoza-Pérez, Felipe</creatorcontrib><creatorcontrib>Gutiérrez-Nava, María Angélica</creatorcontrib><creatorcontrib>Reyes-Pavón, Diana</creatorcontrib><creatorcontrib>Azaola-Espinosa, Alejandro</creatorcontrib><creatorcontrib>Mayorga-Reyes, Lino</creatorcontrib><title>Bifidobacterium longum LBUX23 Isolated from Feces of a Newborn; Potential Probiotic Properties and Genomic Characterization</title><title>Microorganisms (Basel)</title><addtitle>Microorganisms</addtitle><description>is considered a microorganism with probiotic potential, which has been extensively studied, but these probiotic effects are strain dependent. This work aims to characterize the probiotic potential, based on the biochemical and genomic functionality, of
LBUX23, isolated from neonates' feces.
LBUX23 contains one circular genome of 2,287,838 bp with a G+C content of 60.05%, no plasmids, no CRISPR-Cas operon, possesses 56 tRNAs, 9 rRNAs, 1 tmRNA and 1776 coding sequences (CDSs). It has chromosomally encoded resistance genes to ampicillin and dicloxacillin, non-hemolytic activity, and moderate inhibition of
ATCC 25922 and to some emergent pathogen's clinical strains.
LBUX23 was able to utilize lactose, sucrose, fructooligosaccharides (FOS), and lactulose. The maximum peak of bacterial growth was observed in sucrose and FOS at 6 h; in lactose and lactulose, it was shown at 8 h.
LBUX23 can survive in gastrointestinal conditions (pH 4 to 7). A decrease in survival (96.5 and 93.8%) was observed at pH 3 and 3.5 during 120 min.
,
, and
genes could be involved in this tolerance.
LBUX23 can also survive under primary and secondary glyco- or tauro-conjugated bile salts, and a mixture of bile salts due to the high extracellular bile salt hydrolase (BSH) activity (67.3 %), in taurocholic acid followed by taurodeoxycholic acid (48.5%), glycocholic acid (47.1%), oxgall (44.3%), and glycodeoxycholic acid (29.7%) probably due to the presence of the
and
genes which form an operon (start: 119573 and end: 123812). Low BSH activity was determined intracellularly (<7%), particularly in glycocholic acid; no intracellular activity was shown.
LBUX23 showed antioxidant effects in DPPH radical, mainly in intact cells (27.4%). In the case of hydroxyl radical scavenging capacity, cell debris showed the highest reduction (72.5%). In the cell-free extract, superoxide anion radical scavenging capacity was higher (90.5%). The genome of
LBUX23 contains
,
,
,
and
genes, which could be involved in this activity. Regarding adherence, it showed adherence up to 5% to Caco-2 cells.
LBUX23 showed in vitro potential probiotic properties, mainly in BSH activity and antioxidant capacity, which indicates that it could be a good candidate for antioxidant or anti-cholesterol tests using in vivo models.</description><subject>Alzheimer's disease</subject><subject>Ampicillin</subject><subject>Antibiotics</subject><subject>antioxidant</subject><subject>Antioxidants</subject><subject>Bifidobacterium</subject><subject>Bifidobacterium longum</subject><subject>Bile</subject><subject>Bile salts</subject><subject>BSH activity</subject><subject>Cholesterol</subject><subject>CRISPR</subject><subject>Dicloxacillin</subject><subject>E coli</subject><subject>Enzymes</subject><subject>Feces</subject><subject>Fructooligosaccharides</subject><subject>functional characterization</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>genome</subject><subject>Genomics</subject><subject>Hydroxyl radicals</subject><subject>Identification and classification</subject><subject>In vivo methods and tests</subject><subject>Lactose</subject><subject>Lactulose</subject><subject>Neonates</subject><subject>Pathogens</subject><subject>Physiological aspects</subject><subject>Plasmids</subject><subject>Probiotics</subject><subject>Scavenging</subject><subject>Sucrose</subject><subject>Superoxide anions</subject><subject>Survival</subject><subject>Taurocholic acid</subject><subject>Taurodeoxycholic acid</subject><subject>Testing</subject><subject>TmRNA</subject><issn>2076-2607</issn><issn>2076-2607</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptUk1vEzEQXSEQrUr_QmWJC5cUf-zaXnFAbURLpAh6oBI3y2uPU0e7drA3RdA_Xy8ppUG1DzMav_fmw1NVJwSfMtbi94M3Kca00sHnIROCBeG1fFEdUiz4jHIsXj7xD6rjnNe4nJYw2ZDX1QETDaGSksPq7tw7b2OnzQjJbwfUx7AqZnl-_Z0ytMix1yNY5FIc0AUYyCg6pNEX-NnFFD6gqzhCGL3u0VWKnY-jN5O3gTT6AtbBoksIsZSM5jc67fL81qOP4U31yuk-w_GDPaquLz59m3-eLb9eLuZny5lpBB9nTcOMEx2BVpb81nLAHRWS4c51QIXFTAhwNaHUMWMctzUzTFqNjRasdS07qhY7XRv1Wm2SH3T6paL26k-gDFLpUq3pQUkOpOUMbMtxjQ1tJSa6q-tOSsAG86L1cae12XYDWFN6T7rfE91_Cf5GreKtIniavWRF4d2DQoo_tpBHNfhsoO91gLjNisq6xgXaTsne_gddx20KZVYTimHJuGz-oVa6dOCDiyWxmUTVmWha0nBZk4I6fQZVroXyNzGA8yW-R-A7Qtm1nBO4xyYJVtMaqufXsBBPno7okfZ36dg9IJHdJw</recordid><startdate>20230624</startdate><enddate>20230624</enddate><creator>Reyes-Castillo, Pedro A</creator><creator>González-Vázquez, Raquel</creator><creator>Torres-Maravilla, Edgar</creator><creator>Bautista-Hernández, Jessica I</creator><creator>Zúñiga-León, Eduardo</creator><creator>Leyte-Lugo, Martha</creator><creator>Mateos-Sánchez, Leovigildo</creator><creator>Mendoza-Pérez, Felipe</creator><creator>Gutiérrez-Nava, María Angélica</creator><creator>Reyes-Pavón, Diana</creator><creator>Azaola-Espinosa, Alejandro</creator><creator>Mayorga-Reyes, Lino</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-3359-4517</orcidid><orcidid>https://orcid.org/0000-0002-3850-1244</orcidid><orcidid>https://orcid.org/0000-0003-4940-6194</orcidid><orcidid>https://orcid.org/0000-0003-4400-7506</orcidid><orcidid>https://orcid.org/0000-0001-8051-9432</orcidid><orcidid>https://orcid.org/0000-0001-9768-2842</orcidid><orcidid>https://orcid.org/0000-0001-6342-5549</orcidid></search><sort><creationdate>20230624</creationdate><title>Bifidobacterium longum LBUX23 Isolated from Feces of a Newborn; Potential Probiotic Properties and Genomic Characterization</title><author>Reyes-Castillo, Pedro A ; González-Vázquez, Raquel ; Torres-Maravilla, Edgar ; Bautista-Hernández, Jessica I ; Zúñiga-León, Eduardo ; Leyte-Lugo, Martha ; Mateos-Sánchez, Leovigildo ; Mendoza-Pérez, Felipe ; Gutiérrez-Nava, María Angélica ; Reyes-Pavón, Diana ; Azaola-Espinosa, Alejandro ; Mayorga-Reyes, Lino</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c576t-553cf7b1e98ecedd6e0b27830bfbe27d0377ef4122f3ccf6d43c38da0ca739f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Alzheimer's disease</topic><topic>Ampicillin</topic><topic>Antibiotics</topic><topic>antioxidant</topic><topic>Antioxidants</topic><topic>Bifidobacterium</topic><topic>Bifidobacterium longum</topic><topic>Bile</topic><topic>Bile salts</topic><topic>BSH activity</topic><topic>Cholesterol</topic><topic>CRISPR</topic><topic>Dicloxacillin</topic><topic>E coli</topic><topic>Enzymes</topic><topic>Feces</topic><topic>Fructooligosaccharides</topic><topic>functional characterization</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>genome</topic><topic>Genomics</topic><topic>Hydroxyl radicals</topic><topic>Identification and classification</topic><topic>In vivo methods and tests</topic><topic>Lactose</topic><topic>Lactulose</topic><topic>Neonates</topic><topic>Pathogens</topic><topic>Physiological aspects</topic><topic>Plasmids</topic><topic>Probiotics</topic><topic>Scavenging</topic><topic>Sucrose</topic><topic>Superoxide anions</topic><topic>Survival</topic><topic>Taurocholic acid</topic><topic>Taurodeoxycholic acid</topic><topic>Testing</topic><topic>TmRNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reyes-Castillo, Pedro A</creatorcontrib><creatorcontrib>González-Vázquez, Raquel</creatorcontrib><creatorcontrib>Torres-Maravilla, Edgar</creatorcontrib><creatorcontrib>Bautista-Hernández, Jessica I</creatorcontrib><creatorcontrib>Zúñiga-León, Eduardo</creatorcontrib><creatorcontrib>Leyte-Lugo, Martha</creatorcontrib><creatorcontrib>Mateos-Sánchez, Leovigildo</creatorcontrib><creatorcontrib>Mendoza-Pérez, Felipe</creatorcontrib><creatorcontrib>Gutiérrez-Nava, María Angélica</creatorcontrib><creatorcontrib>Reyes-Pavón, Diana</creatorcontrib><creatorcontrib>Azaola-Espinosa, Alejandro</creatorcontrib><creatorcontrib>Mayorga-Reyes, Lino</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DAOJ: Directory of Open Access Journals</collection><jtitle>Microorganisms (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reyes-Castillo, Pedro A</au><au>González-Vázquez, Raquel</au><au>Torres-Maravilla, Edgar</au><au>Bautista-Hernández, Jessica I</au><au>Zúñiga-León, Eduardo</au><au>Leyte-Lugo, Martha</au><au>Mateos-Sánchez, Leovigildo</au><au>Mendoza-Pérez, Felipe</au><au>Gutiérrez-Nava, María Angélica</au><au>Reyes-Pavón, Diana</au><au>Azaola-Espinosa, Alejandro</au><au>Mayorga-Reyes, Lino</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bifidobacterium longum LBUX23 Isolated from Feces of a Newborn; Potential Probiotic Properties and Genomic Characterization</atitle><jtitle>Microorganisms (Basel)</jtitle><addtitle>Microorganisms</addtitle><date>2023-06-24</date><risdate>2023</risdate><volume>11</volume><issue>7</issue><spage>1648</spage><pages>1648-</pages><issn>2076-2607</issn><eissn>2076-2607</eissn><abstract>is considered a microorganism with probiotic potential, which has been extensively studied, but these probiotic effects are strain dependent. This work aims to characterize the probiotic potential, based on the biochemical and genomic functionality, of
LBUX23, isolated from neonates' feces.
LBUX23 contains one circular genome of 2,287,838 bp with a G+C content of 60.05%, no plasmids, no CRISPR-Cas operon, possesses 56 tRNAs, 9 rRNAs, 1 tmRNA and 1776 coding sequences (CDSs). It has chromosomally encoded resistance genes to ampicillin and dicloxacillin, non-hemolytic activity, and moderate inhibition of
ATCC 25922 and to some emergent pathogen's clinical strains.
LBUX23 was able to utilize lactose, sucrose, fructooligosaccharides (FOS), and lactulose. The maximum peak of bacterial growth was observed in sucrose and FOS at 6 h; in lactose and lactulose, it was shown at 8 h.
LBUX23 can survive in gastrointestinal conditions (pH 4 to 7). A decrease in survival (96.5 and 93.8%) was observed at pH 3 and 3.5 during 120 min.
,
, and
genes could be involved in this tolerance.
LBUX23 can also survive under primary and secondary glyco- or tauro-conjugated bile salts, and a mixture of bile salts due to the high extracellular bile salt hydrolase (BSH) activity (67.3 %), in taurocholic acid followed by taurodeoxycholic acid (48.5%), glycocholic acid (47.1%), oxgall (44.3%), and glycodeoxycholic acid (29.7%) probably due to the presence of the
and
genes which form an operon (start: 119573 and end: 123812). Low BSH activity was determined intracellularly (<7%), particularly in glycocholic acid; no intracellular activity was shown.
LBUX23 showed antioxidant effects in DPPH radical, mainly in intact cells (27.4%). In the case of hydroxyl radical scavenging capacity, cell debris showed the highest reduction (72.5%). In the cell-free extract, superoxide anion radical scavenging capacity was higher (90.5%). The genome of
LBUX23 contains
,
,
,
and
genes, which could be involved in this activity. Regarding adherence, it showed adherence up to 5% to Caco-2 cells.
LBUX23 showed in vitro potential probiotic properties, mainly in BSH activity and antioxidant capacity, which indicates that it could be a good candidate for antioxidant or anti-cholesterol tests using in vivo models.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>37512821</pmid><doi>10.3390/microorganisms11071648</doi><orcidid>https://orcid.org/0000-0002-3359-4517</orcidid><orcidid>https://orcid.org/0000-0002-3850-1244</orcidid><orcidid>https://orcid.org/0000-0003-4940-6194</orcidid><orcidid>https://orcid.org/0000-0003-4400-7506</orcidid><orcidid>https://orcid.org/0000-0001-8051-9432</orcidid><orcidid>https://orcid.org/0000-0001-9768-2842</orcidid><orcidid>https://orcid.org/0000-0001-6342-5549</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2076-2607 |
| ispartof | Microorganisms (Basel), 2023-06, Vol.11 (7), p.1648 |
| issn | 2076-2607 2076-2607 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_86e1963ed96040c29801ab44b88e0c06 |
| source | Publicly Available Content (ProQuest); PubMed Central |
| subjects | Alzheimer's disease Ampicillin Antibiotics antioxidant Antioxidants Bifidobacterium Bifidobacterium longum Bile Bile salts BSH activity Cholesterol CRISPR Dicloxacillin E coli Enzymes Feces Fructooligosaccharides functional characterization Genes Genetic aspects genome Genomics Hydroxyl radicals Identification and classification In vivo methods and tests Lactose Lactulose Neonates Pathogens Physiological aspects Plasmids Probiotics Scavenging Sucrose Superoxide anions Survival Taurocholic acid Taurodeoxycholic acid Testing TmRNA |
| title | Bifidobacterium longum LBUX23 Isolated from Feces of a Newborn; Potential Probiotic Properties and Genomic Characterization |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T09%3A37%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bifidobacterium%20longum%20LBUX23%20Isolated%20from%20Feces%20of%20a%20Newborn;%20Potential%20Probiotic%20Properties%20and%20Genomic%20Characterization&rft.jtitle=Microorganisms%20(Basel)&rft.au=Reyes-Castillo,%20Pedro%20A&rft.date=2023-06-24&rft.volume=11&rft.issue=7&rft.spage=1648&rft.pages=1648-&rft.issn=2076-2607&rft.eissn=2076-2607&rft_id=info:doi/10.3390/microorganisms11071648&rft_dat=%3Cgale_doaj_%3EA759156841%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c576t-553cf7b1e98ecedd6e0b27830bfbe27d0377ef4122f3ccf6d43c38da0ca739f93%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2843083685&rft_id=info:pmid/37512821&rft_galeid=A759156841&rfr_iscdi=true |