DNP and ATP regulate the pulp breakdown development in Phomopsis longanae Chi-infected longan fruit through modulating the ROS metabolism

• Published in: Food Chemistry: X 2022-06, Vol.14, p.100348-100348, Article 100348
• Main Authors: Lin, Yuzhao, Lin, Hetong, Zeng, Lingzhen, Zheng, Yi, Chen, Yazhen, Fan, Zhongqi, Lin, Yifen
• Format: Article
• Language: English
• Subjects: (s) from the Special Issue on Oxidation in Food by Lingjun Ma; 2,4-Dinitrophenol (DNP); Adenosine triphosphate (ATP); Longan fruit; Membrane lipids peroxidation; Phomopsis longanae Chi; Pulp breakdown; Reactive oxygen species (ROS); ROS metabolism
• ISSN: 2590-1575; 2590-1575
• DOI: 10.1016/j.fochx.2022.100348

•2,4-Dinitrophenol (DNP) promoted the pulp breakdown in P. longanae-infected longan.•DNP raised O2–. generation rate and MDA level in pulp of P. longanae-infected longan.•DNP lowered DlSODs, DlCAT1 and DlAPX6 transcript level in P. longanae-infected longan.•DNP decreased the ROS-scavenging capacity in pulp of P. longanae-infected longan.•ATP restrained P. longanae-induced pulp breakdown via raising ROS-scavenging capacity. Compared with the P. longanae-infected longan, the DNP-treated P. longanae-infected fruit represented a higher pulp breakdown index, a higher O2–. production rate, and a higher MDA content, but the lower activities of APX, SOD and CAT, the lower transcript levels of DlAPX6, DlSOD1, DlSOD2, DlSOD3 and DlCAT1, the lower values of AsA, GSH, flavonoid and total phenolics, a lower scavenging ability of DPPH radical, and a lower value of reducing power. Whereas, the ATP-treated P. longanae-infected samples showed the contrary results. The above findings indicated that the DNP-promoted the pulp breakdown in P. longanae-infected longan was because DNP weakened the capacity of scavenging ROS, raised the O2–. level, and accelerated the membrane lipids peroxidation. However, the ATP-suppressed the pulp breakdown in P. longanae-infected longan was because ATP improved the capacity of scavenging ROS, reduced the O2–. level, and reduced the membrane lipids peroxidation.