Molecular surveillance of pfcrt, pfmdr1 and pfk13-propeller mutations in Plasmodium falciparum isolates imported from Africa to China

The emergence and spread of multidrug resistance poses a significant risk to malaria control and eradication goals in the world. There has been no indigenous malaria cases reported in China since 2017, and China is approaching national malaria elimination. Therefore, anti-malarial drug resistance su...

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Bibliographic Details
Published in:Malaria journal 2021-02, Vol.20 (1), p.73-73, Article 73
Main Authors: Huang, Fang, Yan, He, Xue, Jing-Bo, Cui, Yan-Wen, Zhou, Shui-Sen, Xia, Zhi-Gui, Abeyasinghe, Rabindra, Ringwald, Pascal, Zhou, Xiao-Nong
Format: Article
Language:English
Subjects:
Africa
Amino acids
Analysis
Artemisinin
China
Codons
Deoxyribonucleic acid
DNA
Drug resistance
Drugs
Emergence
Epidemiology
Erythrocytes
Gene mapping
Gene mutations
Genes
Genetic aspects
Haplotypes
Human diseases
Identification
Malaria
Molecular diagnostic techniques
Molecular markers
Multidrug resistance
Mutants
Mutation
Nucleotide sequence
PCR
Pfmdr1 gene
Plasmodium falciparum
Polymerase chain reaction
Software
Surveillance
Vector-borne diseases
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Summary:The emergence and spread of multidrug resistance poses a significant risk to malaria control and eradication goals in the world. There has been no indigenous malaria cases reported in China since 2017, and China is approaching national malaria elimination. Therefore, anti-malarial drug resistance surveillance and tracking the emergence and spread of imported drug-resistant malaria cases will be necessary in a post-elimination phase in China. Dried blood spots were obtained from Plasmodium falciparum-infected cases returned from Africa to China between 2012 and 2015, prior to anti-malarial drug treatment. Whole DNA were extracted and known polymorphisms relating to drug resistance of pfcrt, pfmdr1 gene, and the propeller domain of pfk13 were evaluated by nested PCR and sequencing. The haplotypes and prevalence of these three genes were evaluated separately. Chi-squared test and Fisher's exact test were used to evaluate differences among the different sub-regions of Africa. A P value 
ISSN:1475-2875
1475-2875
DOI:10.1186/s12936-021-03613-5