Effective SARS-CoV-2 replication of monolayers of intestinal epithelial cells differentiated from human induced pluripotent stem cells

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes severe acute respiratory symptoms in humans. Controlling the coronavirus disease pandemic is a worldwide priority. The number of SARS-CoV-2 studies has dramatically increased, and the requirement for analytical tools is higher than...

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Bibliographic Details
Published in:Scientific reports 2023-07, Vol.13 (1), p.11610-11610, Article 11610
Main Authors: Minami, Shohei, Matsumoto, Naomi, Omori, Hiroko, Nakamura, Yutaka, Tamiya, Shigeyuki, Nouda, Ryotaro, Nurdin, Jeffery A., Yamasaki, Moeko, Kotaki, Tomohiro, Kanai, Yuta, Okamoto, Toru, Tachibana, Taro, Ushijima, Hiroshi, Kobayashi, Takeshi, Sato, Shintaro
Format: Article
Language:English
Subjects:
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ACE2
Angiotensin
Angiotensin-converting enzyme 2
Cell differentiation
Coronaviruses
COVID-19
Disease transmission
Enzymes
Epithelial Cells
Humanities and Social Sciences
Humans
Induced Pluripotent Stem Cells
Infections
Intestine
Intestines
multidisciplinary
Organoids
Pandemics
Peptidyl-dipeptidase A
Pluripotency
Propagation
Protein expression
Proteins
Research centers
SARS-CoV-2
Science
Science (multidisciplinary)
Severe acute respiratory syndrome coronavirus 2
Stem cells
Viruses
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Summary:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes severe acute respiratory symptoms in humans. Controlling the coronavirus disease pandemic is a worldwide priority. The number of SARS-CoV-2 studies has dramatically increased, and the requirement for analytical tools is higher than ever. Here, we propose monolayered-intestinal epithelial cells (IECs) derived from human induced pluripotent stem cells (iPSCs) instead of three-dimensional cultured intestinal organoids as a suitable tool to study SARS-CoV-2 infection. Differentiated IEC monolayers express high levels of angiotensin-converting enzyme 2 and transmembrane protease serine 2 (TMPRSS2), host factors essential for SARS-CoV-2 infection. SARS-CoV-2 efficiently grows in IEC monolayers. Using this propagation system, we confirm that TMPRSS2 inhibition blocked SARS-CoV-2 infection in IECs. Hence, our iPSC-derived IEC monolayers are suitable for SARS-CoV-2 research under physiologically relevant conditions.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-38548-1