The Relationship between Microbial Community Succession and Flavor Formation during the Natural Fermentation of Hongqu sufu

• Published in: Foods 2023-07, Vol.12 (14), p.2800
• Main Authors: Luo, Aiguo, Cheng, Zilong, Zhao, Jia, Hao, Jianwei, Shi, Shengli, Hu, Bianfang
• Format: Article
• Language: English
• Subjects: Alcohols; Aldehydes; Alkanes; Alkenes; Aroma compounds; Bacteria; Benzene; Biotechnology industry; Chromatography; Correlation analysis; Enzymes; Esters; Fermentation; Flavor compounds; Flavors; Flora; Food science; Fungi; Gas chromatography; Hafnium oxide; Headspace; Hongqu sufu; Ketones; Mass spectrometry; Mass spectroscopy; microbial communities; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Next-generation sequencing; Organic compounds; Phenols; Scientific imaging; Solid phase methods; Solid phases; Soy products; Sulfur; Sulfur compounds; VOCs; Volatile organic compounds
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods12142800

To study the diversity of microbial flora in Hongqu sufu and analyze the characteristics of special flavor compounds, this study took self-made Hongqu sufu as the research object. Dynamic changes in sufu during fermentation were studied. High-throughput sequencing (HTS) was used to analyze changes in the diversity of fungal and bacterial communities during fermentation. The results showed that at the phylum level, the dominant fungal phyla were identified, Mucormyces and Ascomycetes. The dominant bacterial phyla were Proteobacteria and Firmicutes. At the genus level, the dominant fungal genera were identified as , and . The dominant bacterial genera were , , , and . Headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) combined with technology that can dynamically change flavor compounds was explored to investigate the correlation between microbiota and flavor compounds. In different stages of fermentation, 75 main volatile organic compounds were identified, including seven alcohols, four acids, 16 alkanes, 14 olefins, seven kinds of aldehydes, two kinds of ketones, 10 kinds of esters, one kind of phenol, one kind of sulfur-containing compound, one benzene, and 12 other compounds. The correlation analysis between flora and flavor compounds showed that the fungi genera and were significantly correlated with most flavor compounds. Bacteria genera including , , and had a strong positive correlation with ethyl oleate.