Effects of Aloe vera Flower Extract and Its Active Constituent Isoorientin on Skin Moisturization via Regulating Involucrin Expression: In Vitro and Molecular Docking Studies

Skin moisturization is very crucial for maintaining the flexibility, viscoelasticity, and differentiation of the epidermis and its deprivation causes several diseases from dry skin to dermatitis. , a miracle plant having diverse medicinal properties including skin moisturization effects. This study...

Full description

Saved in:
Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2021-04, Vol.26 (9), p.2626
Main Authors: Razia, Sultana, Park, Hyunsung, Shin, Eunju, Shim, Kyu-Suk, Cho, Eunae, Kim, Sun-Yeou
Format: Article
Language:English
Subjects:
Aloe
Aloe vera
Aloe vera flower
Constituents
Deprivation
Dermatitis
Differentiation
Enzyme-linked immunosorbent assay
Epidermis
Extracellular signal-regulated kinase
Filaggrin
Flowers & plants
Herbal medicine
High-performance liquid chromatography
Hyaluronic acid
involucrin
isoorientin
Keratinocytes
Liquid chromatography
Molecular docking
Phosphorylation
Protein expression
Protein kinase C
Proteins
Skin
skin barrier function
Skin diseases
skin moisturization
Viscoelasticity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Skin moisturization is very crucial for maintaining the flexibility, viscoelasticity, and differentiation of the epidermis and its deprivation causes several diseases from dry skin to dermatitis. , a miracle plant having diverse medicinal properties including skin moisturization effects. This study investigated for the first time the molecular mechanism targeting skin moisturization effects of the flower and its major active constituent. By treating human epidermal keratinocytes (HaCaT cells) with flower water extract (AFWE), we found that AFWE upregulated epidermal involucrin by activating the expression of protein kinase C, p38, and ERK 1/2. Additionally, it modulated filaggrin, increased aquaporin expression, and hyaluronan synthesis via a balanced regulation of HAS1 and HYAL1 protein. Similarly, it was able to protect UVB-induced photodamage. Western blot analysis, ELISA, and qRT- PCR were performed to evaluate various epidermal differentiation markers and moisturization-related factors on human epidermal keratinocytes (HaCaT cells). TLC and HPLC were used to detect and analyze the chemical constituents. Among them, we found that an active component of flower, isoorientin (IO) has a high binding affinity to all of its targeted proteins such as involucrin, PKC, P38, etc. through molecular docking assay. This study indicated that the flower and its active constituent, IO can be used as a prominent ingredient to enhance skin barrier function and improve its related pathologies.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26092626