The distinct spatiotemporal distribution and effect of feed restriction on mtDNA copy number in broilers

Mitochondrial DNA (mtDNA) copy number reflects the abundance of mitochondria in cells and is dependent on the energy requirements of tissues. We hypothesized that the mtDNA copy number in poultry may change with age and tissue, and feed restriction may affect the growth and health of poultry by chan...

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Bibliographic Details
Published in:Scientific reports 2020-02, Vol.10 (1), p.3240-3240, Article 3240
Main Authors: Zhang, Xiangli, Wang, Ting, Ji, Jiefei, Wang, Huanjie, Zhu, Xinghao, Du, Pengfei, Zhu, Yao, Huang, Yanqun, Chen, Wen
Format: Article
Language:English
Subjects:
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Age
Animal Feed
Animals
Body weight
Body Weight - genetics
Chickens - genetics
Chickens - growth & development
Copy number
Dietary restrictions
DNA, Mitochondrial - genetics
Embryos
Energy requirements
Feeds
Female
Gene Dosage
Genomes
Hatching
Humanities and Social Sciences
Liver
Male
Mitochondrial DNA
multidisciplinary
Muscles
Nutrient deficiency
Organ Size - genetics
Organ Specificity - genetics
Poultry
Science
Science (multidisciplinary)
Skeletal muscle
Spatial distribution
Temporal distribution
Tissues
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Summary:Mitochondrial DNA (mtDNA) copy number reflects the abundance of mitochondria in cells and is dependent on the energy requirements of tissues. We hypothesized that the mtDNA copy number in poultry may change with age and tissue, and feed restriction may affect the growth and health of poultry by changing mtDNA content in a tissue-specific pattern. TaqMan real-time PCR was used to quantify mtDNA copy number using three different segments of the mitochondrial genome ( D-loop , ATP6 , and ND6 ) relative to the nuclear single-copy preproglucagon gene ( GCG ). The effect of sex, age, and dietary restriction (quantitative, energy, and protein restriction) on mtDNA copy number variation in the tissues of broilers was investigated. We found that mtDNA copy number varied among tissues ( P  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-60123-1