Preparation of dry flowable formulations of Clonostachys rosea by spray drying and application for Sclerotinia sclerotiorum control

• Published in: Journal of Integrative Agriculture 2018-03, Vol.17 (3), p.613-620
• Main Authors: WU, Hong-qu, SUN, Li-li, LIU, Fang, WANG, Zhi-ying, CAO, Chuan-wang
• Format: Article
• Language: English
• Subjects: Clonostachys rosea; dry flowable formulation; Sclerotinia sclerotiorum; spray drying; 控制效率; 水花; 流动; 干燥; CMC-Na; 纤维素钠; 物理特征; 杀真菌剂
• ISSN: 2095-3119; 2352-3425
• DOI: 10.1016/S2095-3119(17)61811-2

A dry flowable formulation of Clonostachys rosea with fungicidal activity against Sclerotinia sclerotiorum was prepared by spray drying. The formulation was optimized by a four-factor, three-level orthogonal experiment to screen inert ingredients and spray-drying conditions. The optimal dry flowable formulation of C. rosea included 30% C. rosea （ratio of conidia powder and its fermentation broth is 1：3）, 3% Morwet EFW, 4% K12, 10% Morwet D425, 9% sodium salt of polynaphthalene sulphonic acid （NNO）, 5% croscarmellose sodium, 5% （NH4）2SO4, 0.5% carboxymethyl cellulose sodium （CMC-Na）, 1% oxalic acid and palygorskite （carrier） up to 100%. The formulation exhibited good physical characteristics, such as high dispersibility, viability and a long shelf life. Plate antagonism tests and pot trials indicated that the dry flowable formulation was very effective against S. sclerotiorum, with control efficiency of up to 88.30%. This dry flowable formulation of C. rosea is a new potential commercial fungicide for spray drying to control S. sclerotiorum.