Expression of Mir-21 and Mir-103a in Toxocara canis : Potential for Diagnosis of Human Toxocariasis

Toxocariasis is one of the most neglected zoonotic diseases, predominantly caused by . We aimed to evaluate the expression of microRNAs 21 and 103a in seropositive individuals for human toxocariasis as diagnostic biomarkers. This study was conducted on 324 individuals for ELISA test on toxocariasis...

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Bibliographic Details
Published in:Iranian journal of parasitology 2020-10, Vol.15 (4), p.559-567
Main Authors: Raissi, Vahid, Mohebali, Mehdi, Kia, Eshrat Bigom, Rahimi Foroushani, Abbas, Sohrabi, Nasrin, Rokni, Mohammad Bagher, Zibaei, Mohammad
Format: Article
Language:English
Subjects:
Biomarkers
miRNAs expression
Original
Polymerase chain reac-tion
Toxocariasis
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Summary:Toxocariasis is one of the most neglected zoonotic diseases, predominantly caused by . We aimed to evaluate the expression of microRNAs 21 and 103a in seropositive individuals for human toxocariasis as diagnostic biomarkers. This study was conducted on 324 individuals for ELISA test on toxocariasis in Tehran and Karaj, Iran 2019. Then positive samples for anti- IgG were obtained to quantitative Real-time PCR (qRT-PCR) assays to investigate the transcriptional profiles of miRNAs predicted to be involved in developmental and reproductive processes. qPCR was employed to assess levels of transcription for miRNAs of 103a and 21 in plasma samples. After the experiments, the results were evaluated by REST software, Livak formula and quantitative -test. The analyzes performed on human samples showed that in the case group compared to the control group, only in miR-21 gene, a 0.3-fold increase in expression was obtained with REST software (Fold change ≤ 1.5, >0.05), which was statistically significant by -test (
ISSN:1735-7020
2008-238X
DOI:10.18502/ijpa.v15i4.4865