Kinase Inhibitor VvBKI1 Interacts with Ascorbate Peroxidase VvAPX1 Promoting Plant Resistance to Oomycetes

Downy mildew caused by oomycete pathogen is a devastating disease of grapevine. secretes an array of RXLR effectors to enhance virulence. One of these effectors, PvRXLR131, has been reported to interact with grape ( ) BRI1 kinase inhibitor (VvBKI1). BKI1 is conserved in and . However, the role of Vv...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-03, Vol.24 (6), p.5106
Main Authors: Lv, Junli, Wu, Wei, Ma, Tao, Yang, Bohan, Khan, Asaf, Fu, Peining, Lu, Jiang
Format: Article
Language:English
Subjects:
Abiotic stress
Arabidopsis
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis thaliana
Ascorbate Peroxidases - metabolism
Ascorbic acid
Disease resistance
Disease Resistance - genetics
Downy mildew
Ectopic expression
Effectors
Enzyme inhibitors
Enzymes
Gene Expression Regulation, Plant
Genetic engineering
Grapes
Grapevines
Kinases
L-Ascorbate peroxidase
Localization
oomycete
Oomycetes
Pathogens
Peroxidase
Phytophthora - metabolism
Plant Diseases - genetics
Plant immunity
Plant resistance
Proteins
Proteins - metabolism
resistance
Scavenging
Virulence
Vitis - genetics
Vitis - metabolism
Vitis vinifera
VvAPX1
VvBKI1
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Summary:Downy mildew caused by oomycete pathogen is a devastating disease of grapevine. secretes an array of RXLR effectors to enhance virulence. One of these effectors, PvRXLR131, has been reported to interact with grape ( ) BRI1 kinase inhibitor (VvBKI1). BKI1 is conserved in and . However, the role of VvBKI1 in plant immunity is unknown. Here, we found transient expression of in grapevine and increased its resistance to and , respectively. Furthermore, ectopic expression of VvBKI1 in Arabidopsis can increase its resistance to downy mildew caused by . Further experiments revealed that VvBKI1 interacts with a cytoplasmic ascorbate peroxidase, VvAPX1, an ROS-scavenging protein. Transient expression of in grape and promoted its resistance against , and . Moreover, transgenic Arabidopsis is more resistant to . Furthermore, both VvBKI1 and VvAPX1 transgenic Arabidopsis showed an elevated ascorbate peroxidase activity and enhanced disease resistance. In summary, our findings suggest a positive correlation between APX activity and resistance to oomycetes and that this regulatory network is conserved in , , and .
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24065106