Genomics of Tenacibaculum Species in British Columbia, Canada

is a genus of Gram-negative filamentous bacteria with a cosmopolitan distribution. The research describing genomes stems primarily from Norway and Chile due to their impacts on salmon aquaculture. Canadian salmon aquaculture also experiences mortality events related to the presence of spp., yet no C...

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Bibliographic Details
Published in:Pathogens (Basel) 2023-01, Vol.12 (1), p.101
Main Authors: Nowlan, Joseph P, Sies, Ashton N, Britney, Scott R, Cameron, Andrew D S, Siah, Ahmed, Lumsden, John S, Russell, Spencer
Format: Article
Language:English
Subjects:
Annotations
Antimicrobial agents
Antimicrobial resistance
Aquaculture
Bacteria
Chromosomes
Comparative analysis
de novo assembly
diversity
DNA sequencing
Drug resistance
Filamentous bacteria
Genes
Genomes
Genomics
Gram-negative bacteria
Hemolysins
Metalloproteinase
New species
Nucleotides
Oxytetracycline
Pathogenesis
Pathogens
phylogenetics
Salmon
Secretion
Tenacibaculum
Toxins
Veterinary medicine
Virulence
Whole genome sequencing
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Summary:is a genus of Gram-negative filamentous bacteria with a cosmopolitan distribution. The research describing genomes stems primarily from Norway and Chile due to their impacts on salmon aquaculture. Canadian salmon aquaculture also experiences mortality events related to the presence of spp., yet no Canadian genomes are publicly available. Ribosomal DNA sequencing of 16S and four species-specific 16S quantitative-PCR assays were used to select isolates cultured from Atlantic salmon with mouthrot in British Columbia (BC), Canada. Ten isolates representing four known and two unknown species of were selected for shotgun whole genome sequencing using the Oxford Nanopore's MinION platform. The genome assemblies achieved closed circular chromosomes for seven isolates and long contigs for the remaining three isolates. Average nucleotide identity analysis identified , , , two genomovars of , and two proposed novel species sp. nov. type strain 18-2881-A and sp. nov. type strain 18-3228-7B . Annotation in most of the isolates predicted putative virulence and antimicrobial resistance genes, most-notably toxins (i.e., hemolysins), type-IX secretion systems, and oxytetracycline resistance. Comparative analysis with the type-strain predicted additional toxins and numerous C-terminal secretion proteins, including an M12B family metalloprotease in the isolates from BC. The genomic prediction of virulence-associated genes provides important targets for studies of mouthrot disease, and the annotation of the antimicrobial resistance genes provides targets for surveillance and diagnosis in veterinary medicine.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens12010101