Genotoxic effect of heavy metals on Astyanax lacustris in an urban stream

• Published in: Heliyon 2020-09, Vol.6 (9), p.e05034-e05034, Article e05034
• Main Authors: Silva, Emanoelle Pereira da, Benvindo-Souza, Marcelino, Cotrim, Carlos Filipe Camilo, Motta, Andreya Gonçalves Costa, Lucena, Matheus Mendonça, Antoniosi Filho, Nelson Roberto, Pereira, Julião, Formiga, Klebber Teodomiro Martins, Melo e Silva, Daniela
• Format: Article
• Language: English
• Subjects: Astyanax lacustris; biomarkers; Comet assay; DNA damage; Environmental analysis; Environmental chemistry; Environmental pollution; Environmental science; Environmental toxicology; Fish; genotoxicity; Heavy metals; mutagens; Pollution; sediments; streams; tropical fish; urbanization
• ISSN: 2405-8440; 2405-8440
• DOI: 10.1016/j.heliyon.2020.e05034

Uncontrolled urbanization growth contributes to the pollution of aquatic environments. Heavy metals released by domestic and industrial effluents can negatively affect aquatic organisms. This study aimed to evaluate the effect of environmental pollutants, such as metals, on fish DNA damage, in stretches of an urban stream. Specimens of the Neotropical fish, Astyanax lacustris, were exposed in situ for 96 h along the Antas stream, a Brazilian aquatic system deteriorated by anthropogenic factors. Water and sediment samples were collected simultaneously for physicochemical and heavy metal analyses. The comet assay was performed as a biomarker of genotoxicity. Fish located downstream had a higher frequency of DNA damage than in the reference site. We found concentrations of Cr and Ni above acceptable levels in sediment samples. Generally, Ba, Mn, Mg, Zn, Cr, and Ni were the elements most associated with genotoxic damage. Water and sediment of the Antas stream showed genotoxic potential in A. lacustris according to the urbanization gradient, demonstrating the importance to prevent the release of environmental pollutants, especially heavy metals in urban areas. Environmental science; Environmental analysis; Environmental chemistry; Environmental pollution; Environmental toxicology; Fish; Heavy metals; DNA damage; Pollution; Comet assay