Pursuing Experimental Reproducibility: An Efficient Protocol for the Preparation of Cerebrospinal Fluid Samples for NMR-Based Metabolomics and Analysis of Sample Degradation

NMR-based metabolomics investigations of human biofluids offer great potential to uncover new biomarkers. In contrast to protocols for sample collection and biobanking, procedures for sample preparation prior to NMR measurements are still heterogeneous, thus compromising the comparability of the res...

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Bibliographic Details
Published in:Metabolites 2020-06, Vol.10 (6), p.251
Main Authors: Albrecht, Benjamin, Voronina, Elena, Schipke, Carola, Peters, Oliver, Parr, Maria Kristina, Díaz-Hernández, M. Dolores, Schlörer, Nils E.
Format: Article
Language:English
Subjects:
Cerebrospinal fluid
cerebrospinal fluid (CSF)
Communication
Degradation
Ethanol
Identification
Metabolites
Metabolomics
NMR
NMR metabolomics
Nuclear magnetic resonance
Potassium
Proteins
Reproducibility
sample degradation
sample preparation
Spectrum analysis
Studies
time and temperature dependence
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Summary:NMR-based metabolomics investigations of human biofluids offer great potential to uncover new biomarkers. In contrast to protocols for sample collection and biobanking, procedures for sample preparation prior to NMR measurements are still heterogeneous, thus compromising the comparability of the resulting data. Herein, we present results of an investigation of the handling of cerebrospinal fluid (CSF) samples for NMR metabolomics research. Origins of commonly observed problems when conducting NMR experiments on this type of sample are addressed, and suitable experimental conditions in terms of sample preparation and pH control are discussed. Sample stability was assessed by monitoring the degradation of CSF samples by NMR, hereby identifying metabolite candidates, which are potentially affected by sample storage. A protocol was devised yielding consistent spectroscopic data as well as achieving overall sample stability for robust analysis. We present easy to adopt standard operating procedures with the aim to establish a shared sample handling strategy that facilitates and promotes inter-laboratory comparison, and the analysis of sample degradation provides new insights into sample stability.
ISSN:2218-1989
2218-1989
DOI:10.3390/metabo10060251