CRISPR/Cas9: a powerful tool in colorectal cancer research

Colorectal cancer (CRC) is one of the most common malignant cancers worldwide and seriously threatens human health. The clustered regulatory interspaced short palindromic repeat/CRISPR-associate nuclease 9 (CRISPR/Cas9) system is an adaptive immune system of bacteria or archaea. Since its introducti...

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Bibliographic Details
Published in:Journal of experimental & clinical cancer research 2023-11, Vol.42 (1), p.1-308, Article 308
Main Authors: Hu, Yang, Liu, Liang, Jiang, Qi, Fang, Weiping, Chen, Yazhu, Hong, Yuntian, Zhai, Xiang
Format: Article
Language:English
Subjects:
Cancer
Cancer research
Cancer therapies
Cell culture
Colorectal cancer
CRC
CRISPR
CRISPR/Cas9
DNA repair
Drug delivery systems
Drug resistance
Drugs
Gene editing tool
Gene therapy
Genes
Genomes
Genomics
Immune system
Immunotherapy
Medical prognosis
Medical research
Mice
Mutation
Off-target
Oncology, Experimental
Precise medicine
Target therapy
Vehicles
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Summary:Colorectal cancer (CRC) is one of the most common malignant cancers worldwide and seriously threatens human health. The clustered regulatory interspaced short palindromic repeat/CRISPR-associate nuclease 9 (CRISPR/Cas9) system is an adaptive immune system of bacteria or archaea. Since its introduction, research into various aspects of treatment approaches for CRC has been accelerated, including investigation of the oncogenes, tumor suppressor genes (TSGs), drug resistance genes, target genes, mouse model construction, and especially in genome-wide library screening. Furthermore, the CRISPR/Cas9 system can be utilized for gene therapy for CRC, specifically involving in the molecular targeted drug delivery or targeted knockout in vivo. In this review, we elucidate the mechanism of the CRISPR/Cas9 system and its comprehensive applications in CRC. Additionally, we discussed the issue of off-target effects associated with CRISPR/Cas9, which serves to restrict its practical application. Future research on CRC should in-depth and systematically utilize the CRISPR/Cas9 system thereby achieving clinical practice.
ISSN:1756-9966
0392-9078
1756-9966
DOI:10.1186/s13046-023-02901-z