2349 The role of interleukin-23 in human melanoma

OBJECTIVES/SPECIFIC AIMS: Interleukin-23 (IL-23) promotes differentiation of naïve T-cells into Th17 cells, which drive the pathogenesis of autoinflammatory conditions such as psoriasis. IL-23-neutralizing antibody therapies are now in use for treatment of psoriasis, with promising results. Studies...

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Bibliographic Details
Published in:Journal of clinical and translational science 2018-06, Vol.2 (S1), p.32-32
Main Authors: Jani, Aditi, Chaudhary, Sandeep, Janjetovic, Zorica, Sherwani, Mohammad A., Yusuf, Nabiha, Slominski, Andrzej, Athar, Mohammad, Elmets, Craig
Format: Article
Language:English
Subjects:
Antibodies
Basic/Translational Science/Team Science
Cell proliferation
Cytokines
DNA repair
Flow cytometry
Helper cells
Immunofluorescence
Immunotherapy
Interleukin 23
Lymphocytes T
Melanocytes
Melanoma
Metastases
Neoplasia
Patients
Population studies
Psoriasis
Tumorigenicity
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Summary:OBJECTIVES/SPECIFIC AIMS: Interleukin-23 (IL-23) promotes differentiation of naïve T-cells into Th17 cells, which drive the pathogenesis of autoinflammatory conditions such as psoriasis. IL-23-neutralizing antibody therapies are now in use for treatment of psoriasis, with promising results. Studies in mice have shown that IL-23 plays a role in inhibiting the growth, progression, and metastasis of melanomas. Thus, therapeutic neutralization of IL-23 in patients may inadvertently increase their susceptibility to development of melanoma. In this study, we aim to characterize expression of IL-23 receptors (IL-23R) in human melanocytes and melanoma cells and tissue and to study the effect of IL-23 on growth, proliferation, and tumorigenicity of these cells. METHODS/STUDY POPULATION: IL-23R expression was characterized using immunofluorescence staining, Western blot, and flow cytometric analysis. Response of melanoma and melanocytes to recombinant IL-23 treatment will be studied through similar methods in addition to assays of cell proliferation and tumorigenicity. RESULTS/ANTICIPATED RESULTS: Preliminary immunofluorescence staining and flow cytometry results indicate that both human melanoma and primary melanocytes express IL-23 receptors. Western blot analysis showed that melanoma cell line A375 expressed nearly twice the amount of IL-23R versus normal melanocytes ( p
ISSN:2059-8661
2059-8661
DOI:10.1017/cts.2018.135