Analysis of Staphylococcus aureus Molecules in Non-Treated Blood Using Mercury Immobilized Carbon Nanotube Sensor

Staphylococcus aureus bacteria is a ubiquitous Gram-positive microorganism that causes infections related to the sudden infant death syndrome. Recently, basic detection methods depend on complicated PCR amplification, electric separation, spectric adsorption and other detection systems. However, in...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2022-03, Vol.27 (6), p.1837
Main Authors: Cho, In Hea, Choi, Kwang Jin, Kim, Ji Hyun, Lee, Kyung, Ly, Suw Young
Format: Article
Language:English
Subjects:
assay
Assaying
Biosensing Techniques - methods
Blood
Carbon
carbon nanotube sensor
Chemical vapor deposition
Diagnostic systems
Electrodes
Electrolytes
Gram-positive bacteria
Humans
Infections
Mercury
mercury immobilization
molecular probe
Nanotubes, Carbon
SIDS
Staphylococcal Infections
Staphylococcus aureus
Sudden infant death syndrome
Voltammetry
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Summary:Staphylococcus aureus bacteria is a ubiquitous Gram-positive microorganism that causes infections related to the sudden infant death syndrome. Recently, basic detection methods depend on complicated PCR amplification, electric separation, spectric adsorption and other detection systems. However, in this study, simplified sensitive voltammetric skills are developed. To identify an effective diagnostic method for Staphylococcus aureus (SA), a voltammetric sensing probe was sought using mercury immobilized on a carbon nanotube sensor (MCN). The voltammetric MCN conditions were optimized through stripping and cyclic voltammetry. Diagnostic electrolyte was used on non-treated blood sera as an electrolyte solution. The optimum cyclic and stripping analytical working range was 0.5-4.0 mL (3 × 10 ~5 × 10 CFU/0.5 mL) SA. The statistic relative standard deviation of 0.1 mL SA was observed to be 0.0078 (n = 5). Using the optimum parameters, a diagnostic test was performed by the direct assay of SA in non-treated human blood and patient sera. Here, the developed results can be used for the direct assay of non-treated blood sera, organ monitoring, in-vivo diagnosis, and other assays requiring SA detection.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules27061837