Embryonic Exposure to Bisphenol A Impairs Primordial Germ Cell Migration without Jeopardizing Male Breeding Capacity

A large amount of chemicals are released to the environment each year. Among them, bisphenol A (BPA) is of utmost concern since it interferes with the reproductive system of wild organisms due to its capacity to bind to hormone receptors. Additionally, BPA epigenotoxic activity is known to affect ba...

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Bibliographic Details
Published in:Biomolecules (Basel, Switzerland) Switzerland), 2019-07, Vol.9 (8), p.307-14
Main Authors: Lombó, Marta, Getino-Álvarez, Lidia, Depincé, Alexandra, Labbé, Catherine, Herráez, María Paz
Format: Article
Language:English
Subjects:
Animals
Benzhydryl Compounds - pharmacology
bisphenol A
Breeding
Cell Movement - drug effects
Chemokine CXCL12 - genetics
Computer Science
Embryonic Germ Cells - cytology
Embryonic Germ Cells - drug effects
Epigenesis, Genetic - drug effects
epigenetics
Fertility - drug effects
Gene Expression Regulation, Developmental - drug effects
Life Sciences
Male
Phenols - pharmacology
primordial germ cells
Receptors, CXCR4 - genetics
reproduction
Testis - drug effects
Testis - growth & development
zebrafish
Zebrafish - embryology
Zebrafish - genetics
Zebrafish Proteins - genetics
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Summary:A large amount of chemicals are released to the environment each year. Among them, bisphenol A (BPA) is of utmost concern since it interferes with the reproductive system of wild organisms due to its capacity to bind to hormone receptors. Additionally, BPA epigenotoxic activity is known to affect basic processes during embryonic life. However, its effects on primordial germ cells (PGCs) proliferation and migration, both mechanisms being crucial for gametogenesis, remain unknown. To investigate the effects of BPA on PGCs migration and eventual testicle development, zebrafish embryos were exposed to 100, 2000 and 4000 µg/L BPA during the first 24 h of development. Vasa immunostaining of PGCs revealed that exposure to 2000 and 4000 µg/L BPA impaired their migration to the genital ridge. Two pivotal genes of PGCs migration ( and ) were highly dysregulated in embryos exposed to these doses, whereas DNA methylation and epigenetic marks in PGCs and their surrounding somatic cells were not altered. Once embryos reached adulthood, the morphometric study of their gonads revealed that, despite the reduced number of PGCs which colonized the genital ridges, normal testicles were developed. Although H3K9ac decreased in the sperm from treated fishes, it did not affect the progeny development.
ISSN:2218-273X
2218-273X
DOI:10.3390/biom9080307