Reversible photocontrol of oxidase activity by inserting a photosensitive domain into the oxidase

Background Photocontrol of protein activity has become a helpful strategy for regulating biological pathways. Herein, a method for the precise and reversible photocontrol of oxidase activity was developed by using the conformational change of the AsLOV2 domain. Results The AsLOV2 domain was inserted...

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Bibliographic Details
Published in:Bioresources and bioprocessing 2019-08, Vol.6 (1), p.1-6, Article 28
Main Authors: Sun, Tongjing, Zhang, Baoqi, Lin, Jinping, Ren, Yuhong
Format: Article
Language:English
Subjects:
AsLOV2
Biochemical Engineering
Chemistry
Chemistry and Materials Science
Environmental Engineering/Biotechnology
Enzymatic activity
Enzyme activity
Fusion protein
Industrial and Production Engineering
Insertion
Light
Light intensity
Luminous intensity
Metabolic pathways
Optimization
Oxidase
Photocontrol
Photoinhibition
Photosensitivity
Proteins
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Summary:Background Photocontrol of protein activity has become a helpful strategy for regulating biological pathways. Herein, a method for the precise and reversible photocontrol of oxidase activity was developed by using the conformational change of the AsLOV2 domain. Results The AsLOV2 domain was inserted into the nonconserved sites exposed on the surface of the AdhP protein, and the alov9 fusion was successfully screened for subsequent optical experiments under the assumption that neither of these actions affected the original activity of AdhP protein. The activity of alov9 was noticeably inhibited when the fusion was exposed to 470 nm blue light and recovered within 30 min. As a result, we could precisely and reversibly photocontrol alov9 activity through the optimization of several parameters, including cofactor concentration, light intensity, and illumination time. Conclusions An efficient method was developed for the photoinhibition of enzymatic activity based on the insertion of the light-sensitive AsLOV2 domain, providing new ideas for photocontrolling metabolic pathways without carriers in the future.
ISSN:2197-4365
2197-4365
DOI:10.1186/s40643-019-0263-7