Immunomagnetic Separation of Microorganisms with Iron Oxide Nanoparticles

The early detection of Legionella in water reservoirs, and the prevention of their often fatal diseases, requires the development of rapid and reliable detection processes. A method for the magnetic separation (MS) of Legionella pneumophila by superparamagnetic iron oxide nanoparticles is developed,...

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Bibliographic Details
Published in:Chemosensors 2020-03, Vol.8 (1), p.17
Main Authors: Thomas, Julian A., Schnell, Florian, Kaveh-Baghbaderani, Yasmin, Berensmeier, Sonja, Schwaminger, Sebastian P.
Format: Article
Language:English
Subjects:
antibody immobilization
Chemical properties
Control
immunoassay
Immunosuppression
iron oxide nanoparticles
Iron oxides
magnetic nanoparticles
Magnetic separation
Methods
microorganism detection
Microorganisms
nanoparticle functionalization
Nanoparticles
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Summary:The early detection of Legionella in water reservoirs, and the prevention of their often fatal diseases, requires the development of rapid and reliable detection processes. A method for the magnetic separation (MS) of Legionella pneumophila by superparamagnetic iron oxide nanoparticles is developed, which represents the basis for future bacteria detection kits. The focus lies on the separation process and the simplicity of using magnetic nanomaterials. Iron oxide nanoparticles are functionalized with epoxy groups and Legionella-specific antibodies are immobilized. The resulting complexes are characterized with infrared spectroscopy and tested for the specific separation and enrichment of the selected microorganisms. The cell-particle complexes can be isolated in a magnetic field and detected with conventional methods such as fluorescence detection. A nonspecific enrichment of bacteria is also possible by using bare iron oxide nanoparticles (BIONs), which we used as a reference to the nanoparticles with immobilized antibodies. Furthermore, the immunomagnetic separation can be applied for the detection of multiple other microorganisms and thus might pave the way for simpler bacterial diagnosis.
ISSN:2227-9040
2227-9040
DOI:10.3390/chemosensors8010017