Hearing loss and its association with the proteome of perilymph, cerebrospinal fluid, and tumor tissue in patients with vestibular schwannoma

• Published in: Scientific reports 2024-06, Vol.14 (1), p.14118-13, Article 14118
• Main Authors: Edvardsson Rasmussen, Jesper, Li, Peng, Laurell, Göran, Bergquist, Jonas, Eriksson, Per Olof
• Format: Article
• Language: English
• Subjects: 631/378/2619/1387; 631/378/2619/1592; 692/617/375/364; Adult; Aged; Audiometry, Pure-Tone; Biopsy; Cerebrospinal fluid; Cerebrospinal Fluid - metabolism; CFHR2; Cochlea; Complement component factor H related protein 2; Complement factor H; Female; Hearing loss; Hearing Loss - cerebrospinal fluid; Human; Humanities and Social Sciences; Humans; inflammation; Inner ear; Leukocyte migration; Liquid chromatography; Male; Mass spectrometry; Mass spectroscopy; Meningioma; Middle Aged; multidisciplinary; Neurokirurgi; Neuroma, Acoustic - cerebrospinal fluid; Neuroma, Acoustic - metabolism; Neuroma, Acoustic - pathology; Neurosurgery; Oto-rhino-laryngologi; Oto-Rhino-Laryngology; Perilymph; Perilymph - metabolism; Proteome; Proteomes; Schwann cells; Science; Science (multidisciplinary); Tumors; Up-regulation; vestibular schannoma; Vestibular schwannoma; Vestibular system
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-64352-6

This study examined the association between hearing loss in sporadic vestibular schwannoma patients and the proteome of perilymph (PL), cerebrospinal fluid (CSF), and vestibular schwannoma. Intraoperative sampling of PL and of CSF, and biopsy of vestibular schwannoma tissue, was performed in 32, 32, and 20 patients with vestibular schwannoma, respectively. Perilymph and CSF in three patients with meningioma and normal hearing were also sampled. The proteomes were identified by liquid chromatography coupled to high-resolution tandem mass spectrometry. Preoperative hearing function of the patients was evaluated with pure tone audiometry, with mean values at frequencies of 500, 1000, 2000, and 4000 Hz (PTA4) in the tumor-affected ear used to delineate three hearing groups. Analysis of the PL samples revealed significant upregulation of complement factor H-related protein 2 (CFHR2) in patients with severe to profound hearing loss after false discovery rate correction. Pathway analysis of biofunctions revealed higher activation scores in the severe/profound hearing loss group of leukocyte migration, viral infection, and migration of cells in PL. Upregulation of CFHR2 and activation of these pathways indicate chronic inflammation in the cochlea of vestibular schwannoma patients with severe to profound hearing loss compared with patients with normal hearing or mild hearing loss.