In vitro shoot regeneration from seedling explants in Brassica vegetables: red cabbage, broccoli, Savoy cabbage and cauliflower

Brassica oleracea varieties (red cabbage, broccoli, Savoy cabbage, and cauliflower) were tested for their ability to regenerate shoots in vitro. Cotyledon, hypocotyl and root explants of 7-day old seedlings were incubated on Murashige and Skoog (MS) medium supplemented with 1 mg/l 6-benzyladenine (B...

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Bibliographic Details
Published in:Archives of biological sciences 2010, Vol.62 (2), p.337-345
Main Authors: Pavlović, S., Institute for Vegetable Crops, Smederevska Palanka (Serbia), Vinterhalter, B., Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia), Mitić, N., Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia), Adžic, S., Institute for Vegetable Crops, Smederevska Palanka (Serbia), Pavlović, N., Institute for Vegetable Crops, Smederevska Palanka (Serbia), Zdravković, M., Institute for Vegetable Crops, Smederevska Palanka (Serbia), Vinterhalter, D., Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia)
Format: Article
Language:English
Subjects:
ADAPTACION
ADAPTATION
BRASSICA
Brassica oleracea
CULTIVO IN VITRO
CULTURE IN VITRO
CULTURE MEDIA
cytokinins
EXPLANT
EXPLANTES
EXPLANTS
http://www.fao.org/aos/agrovoc#c_10204
http://www.fao.org/aos/agrovoc#c_1060
http://www.fao.org/aos/agrovoc#c_117
http://www.fao.org/aos/agrovoc#c_24135
http://www.fao.org/aos/agrovoc#c_27520
http://www.fao.org/aos/agrovoc#c_34114
http://www.fao.org/aos/agrovoc#c_5968
http://www.fao.org/aos/agrovoc#c_6945
IN VITRO CULTURE
IN VITRO REGENERATION
MEDIO DE CULTIVO
MILIEU DE CULTURE
plant acclimation
PLANT GROWTH SUBSTANCES
PLANTULAS
PLANTULE
REGENERACION IN VITRO
regeneration
REGENERATION IN VITRO
seedling explants
SEEDLINGS
shoot multiplication
SUBSTANCE DE CROISSANCE VEGETALE
SUSTANCIAS DE CRECIMIENTO VEGETAL
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Summary:Brassica oleracea varieties (red cabbage, broccoli, Savoy cabbage, and cauliflower) were tested for their ability to regenerate shoots in vitro. Cotyledon, hypocotyl and root explants of 7-day old seedlings were incubated on Murashige and Skoog (MS) medium supplemented with 1 mg/l 6-benzyladenine (BA) or 6-furfurylaminopurine (KIM) in combination with 0, 0.1, and 0.2 mg/l indole-3-butyril acid (IBA). Hypocotyls showed the best explants in almost all varieties tested with a minimum regeneration potential of 75% and producing 3.5-7.4 shoots per explant. The BA-supplemented media were optimal for both shoot regeneration and multiplication. Shoots rooted maximally (100%) on plant growth regulator-free MS medium containing 2% or 4% sucrose. Increased sucrose content improved plant acclimation in the greenhouse.
ISSN:0354-4664
1821-4339
DOI:10.2298/ABS1002337P