Bioactive silver nanoparticles fabricated using Lasiurus scindicus and Panicum turgidum seed extracts: anticancer and antibacterial efficiency

• Published in: Scientific reports 2024-02, Vol.14 (1), p.4162-15, Article 4162
• Main Authors: Alburae, Najla, Alshamrani, Rahma, Mohammed, Afrah E.
• Format: Article
• Language: English
• Subjects: 631/61; 631/80; Anti-Bacterial Agents - chemistry; Antibacterial activity; Bio nanotechnology; Biological activity; Biomedicine; Breast cancer; Cancer; Cell damage; Colorectal cancer; Colorectal carcinoma; Humanities and Social Sciences; Lasiurus scindicus; Metal Nanoparticles - chemistry; multidisciplinary; Nanoparticles; Nanotechnology; Panicum - metabolism; Panicum turgidum; Plant extracts; Plant Extracts - chemistry; Science; Science (multidisciplinary); Seeds; Seeds - metabolism; Silver; Silver - chemistry; Spectroscopy, Fourier Transform Infrared; Tumor cell lines; Ultrastructural changes
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-54449-3

Applying extracts from plants is considered a safe approach in biomedicine and bio-nanotechnology. The present report is considered the first study that evaluated the seeds of Lasiurus scindicus and Panicum turgidum as biogenic agents in the synthesis of silver nanoparticles (AgNPs) which had bioactivity against cancer cells and bacteria. Assessment of NPs activity against varied cell lines (colorectal cancer HCT116 and breast cancer MDA MBA 231 and MCF 10A used as control) was performed beside the antibacterial efficiency. Different techniques (DLS, TEM, EDX and FTIR) were applied to characterize the biosynthesized AgNPs. The phytochemicals from both L. scindicus and Panicum turgidum were identified by GC–MS analysis. Spherical monodisperse NPs at average diameters of 149.6 and 100.4 nm were obtained from seed extract of L. scindicus (L-AgNPs) and P. turgidum , (P-AgNPs) respectively. A strong absorption peak at 3 keV is observed by the EDX spectrum in the tested NPs. Our study provided effective NPs in mitigating the tested cell lines and the lowest IC 50 were 7.8 and 10.30 for MDA MB231 treated by L-AgNPs and P-AgNPs, respectively. Both fabricated NPs might differentially target the MDA MB231 cells compared to HCT116 and MCF10A. Ultrastructural changes and damage for the NPs-treated MDA MB231 cells were studied using TEM and LSM analysis. Antibacterial activity was also observed. About 200 compounds were identified in L. scindicus and P. turgidum by GC–MS analysis might be responsible for the NPs reduction and capping abilities. Efficient NPs against cancer cells and microbes were obtained, however large-scale screening is needed to validate our findings.