A Mouse-Based Method to Monitor Wheat Allergens in Novel Wheat Lines and Varieties Created by Crossbreeding: Proof-of-Concept Using Durum and A. tauschii Wheats

Wheat allergies are potentially life-threatening because of the high risk of anaphylaxis. Wheats belong to four genotypes represented in thousands of lines and varieties. Monitoring changes to wheat allergens is critical to prevent inadvertent ntroduction of hyper-allergenic varieties via breeding....

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Bibliographic Details
Published in:International journal of molecular sciences 2022-06, Vol.23 (12), p.6505
Main Authors: Jorgensen, Rick, Raghunath, Rajsri, Gao, Haoran, Olson, Eric, Ng, Perry K. W., Gangur, Venu
Format: Article
Language:English
Subjects:
Allergens
Anaphylaxis
Antibodies
Asthma
Cross-breeding
Disease
Food allergies
Genotypes
Immunoglobulin E
Medical research
mouse model
Plant breeding
Proteins
Public health
Triticum durum
Western blotting
Wheat
wheat allergens
wheat allergy
wheat breeding
wheat genotype
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Summary:Wheat allergies are potentially life-threatening because of the high risk of anaphylaxis. Wheats belong to four genotypes represented in thousands of lines and varieties. Monitoring changes to wheat allergens is critical to prevent inadvertent ntroduction of hyper-allergenic varieties via breeding. However, validated methods for this purpose are unavailable at present. As a proof-of-concept study, we tested the hypothesis that salt-soluble wheat allergens in our mouse model will be identical to those reported for humans. Groups of Balb/cJ mice were rendered allergic to durum wheat salt-soluble protein extract (SSPE). Using blood from allergic mice, a mini hyper-IgE plasma bank was created and used in optimizing an IgE Western blotting (IEWB) to identify IgE binding allergens. The LC-MS/MS was used to sequence the allergenic bands. An ancient Aegilops tauschii wheat was grown in our greenhouse and extracted SSPE. Using the optimized IEWB method followed by sequencing, the cross-reacting allergens in A. tauschii wheat were identified. Database analysis showed all but 2 of the durum wheat allergens and all A. tauschii wheat allergens identified in this model had been reported as human allergens. Thus, this model may be used to identify and monitor potential changes to salt-soluble wheat allergens caused by breeding.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23126505