Phlebotomus papatasi SP15: mRNA expression variability and amino acid sequence polymorphisms of field populations

The Phlebotomus papatasi salivary protein PpSP15 was shown to protect mice against Leishmania major, suggesting that incorporation of salivary molecules in multi-component vaccines may be a viable strategy for anti-Leishmania vaccines. Here, we investigated PpSP15 predicted amino acid sequence varia...

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Bibliographic Details
Published in:Parasites & vectors 2015-05, Vol.8 (1), p.298-298, Article 298
Main Authors: Ramalho-Ortigão, Marcelo, Coutinho-Abreu, Iliano V, Balbino, Valdir Q, Figueiredo, Jr, Carlos Alberto S, Mukbel, Rami, Dayem, Hussan, Hanafi, Hanafi A, El-Hossary, Shabaan S, Fawaz, Emad El-Din Y, Abo-Shehada, Mahmoud, Hoel, David F, Stayback, Gwen, Wadsworth, Mariha, Shoue, Douglas A, Abrudan, Jenica, Lobo, Neil F, Mahon, Andrew R, Emrich, Scott J, Kamhawi, Shaden, Collins, Frank H, McDowell, Mary Ann
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
Antigenic determinants
Comparative analysis
Expression variability
Genetic aspects
Genetic Variation
Insect Proteins - chemistry
Insect Proteins - genetics
Insect Proteins - metabolism
Leishmaniasis
Messenger RNA
Molecular Sequence Data
Phlebotomus - chemistry
Phlebotomus - genetics
Phlebotomus - metabolism
Polymorphism, Genetic
PpSP15
Prevention
RNA, Messenger - genetics
RNA, Messenger - metabolism
Saliva
Sand fly
Sequence Alignment
T cells
Vaccine
Vaccines
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Summary:The Phlebotomus papatasi salivary protein PpSP15 was shown to protect mice against Leishmania major, suggesting that incorporation of salivary molecules in multi-component vaccines may be a viable strategy for anti-Leishmania vaccines. Here, we investigated PpSP15 predicted amino acid sequence variability and mRNA profile of P. papatasi field populations from the Middle East. In addition, predicted MHC class II T-cell epitopes were obtained and compared to areas of amino acid sequence variability within the secreted protein. The analysis of PpSP15 expression from field populations revealed significant intra- and interpopulation variation.. In spite of the variability detected for P. papatasi populations, common epitopes for MHC class II binding are still present and may potentially be used to boost the response against Le. major infections. Conserved epitopes of PpSP15 could potentially be used in the development of a salivary gland antigen-based vaccine.
ISSN:1756-3305
1756-3305
DOI:10.1186/s13071-015-0914-2