The CD133 and CD34 cell types in human umbilical cord blood have the capacity to produce infectious dengue virus particles

Although dengue virus (DENV) can establish infections in hematopoietic stem progenitor cells (HSPCs), there is little information on dengue virus persistent infection in CD34+ and CD133+ cell surface glycoproteins of hematopoietic stem cells (HSCs). CD34 and CD133 also function as cell–cell adhesion...

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Bibliographic Details
Published in:Scientific reports 2023-06, Vol.13 (1), p.10513-10513, Article 10513
Main Authors: Vats, Amrita, Ho, Tzu-Chuan, Puc, Irwin, Chang, Chiung-Hsin, Perng, Guey-Chuen, Chen, Po-Lin
Format: Article
Language:English
Subjects:
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CD34 antigen
Cell adhesion
Cell culture
Cell surface
Confocal microscopy
Cord blood
Dengue fever
Embedding
Flow cytometry
Glycoproteins
Hematopoietic stem cells
Hemopoiesis
Humanities and Social Sciences
Infections
multidisciplinary
Persistent infection
Plaque assay
Progenitor cells
Science
Science (multidisciplinary)
Stem cells
Umbilical cord
Vector-borne diseases
Vero cells
Viruses
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Summary:Although dengue virus (DENV) can establish infections in hematopoietic stem progenitor cells (HSPCs), there is little information on dengue virus persistent infection in CD34+ and CD133+ cell surface glycoproteins of hematopoietic stem cells (HSCs). CD34 and CD133 also function as cell–cell adhesion factors, which are present in umbilical cord blood (UCB). In this study, we sought to establish a persistent infection model of DENV infection in UCB using a prolonged period of infection lasting 30 days. Post-infection, the results exhibited a productive and non-productive phase of DENV production. Using a plaque assay, Western blot, and confocal microscopy, we demonstrated that CD133 and CD34 cells are target cells for DENV infection. Moreover, we showed that DENV particles can be recovered from the non-productive phase of DENV-infected CD34 and CD133 cells after co-incubation with Vero cells. We concluded that CD133 and CD34 retain their capacity to produce the infectious virus due to proliferation and their ability to repopulate, as deduced from a BrdU proliferation assay and flow cytometry analysis using t-distributed stochastic neighbor embedding. In summary, the platform to co-culture infected primitive HSCs from their non-productive phase onto Vero cells will give new insights into understanding the DENV dynamics in cell-to-cell transmission and reactivation of the virus.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-37707-8