Strategies for Proteome-Wide Quantification of Glycosylation Macro- and Micro-Heterogeneity

Protein glycosylation governs key physiological and pathological processes in human cells. Aberrant glycosylation is thus closely associated with disease progression. Mass spectrometry (MS)-based glycoproteomics has emerged as an indispensable tool for investigating glycosylation changes in biologic...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-01, Vol.23 (3), p.1609
Main Authors: Fang, Pan, Ji, Yanlong, Oellerich, Thomas, Urlaub, Henning, Pan, Kuan-Ting
Format: Article
Language:English
Subjects:
Algorithms
Biological properties
Biomarkers
Breast cancer
Disease Progression
Glycopeptides
Glycoproteins - analysis
glycoproteomics
Glycosylation
Heterogeneity
Humans
Isotope Labeling
label free
Mass spectrometry
Mass spectroscopy
Peptides
Prostate cancer
Proteins
Proteomes
Proteomics
Proteomics - methods
quantification
Reagents
Review
Scientific imaging
Software
stable-isotope labeling
Stem cells
Tandem Mass Spectrometry
Therapeutic targets
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Summary:Protein glycosylation governs key physiological and pathological processes in human cells. Aberrant glycosylation is thus closely associated with disease progression. Mass spectrometry (MS)-based glycoproteomics has emerged as an indispensable tool for investigating glycosylation changes in biological samples with high sensitivity. Following rapid improvements in methodologies for reliable intact glycopeptide identification, site-specific quantification of glycopeptide macro- and micro-heterogeneity at the proteome scale has become an urgent need for exploring glycosylation regulations. Here, we summarize recent advances in N- and O-linked glycoproteomic quantification strategies and discuss their limitations. We further describe a strategy to propagate MS data for multilayered glycopeptide quantification, enabling a more comprehensive examination of global and site-specific glycosylation changes. Altogether, we show how quantitative glycoproteomics methods explore glycosylation regulation in human diseases and promote the discovery of biomarkers and therapeutic targets.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23031609