Downstream of GA4, PbCYP78A6 participates in regulating cell cycle-related genes and parthenogenesis in pear (Pyrus bretshneideri Rehd.)

Parthenocarpy results in traits attractive to both consumers and breeders, and it overcomes the obstacle of self-incompatibility in the fruit set of horticultural crops, including pear (Pyrus bretshneider). However, there is limited knowledge regarding the genetic and molecular mechanisms that regul...

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Published in:BMC plant biology 2021-06, Vol.21 (1), p.1-292, Article 292
Main Authors: Zhang, Haiqi, Han, Wei, Wang, Huibin, Cong, Liu, Zhai, Rui, Yang, Chengquan, Wang, Zhigang, Xu, Lingfei
Format: Article
Language:English
Subjects:
Cell cycle
Cell division
Cell growth
Cellular control mechanisms
Crop production
Cyclin-dependent kinases
Cytochrome
Cytochrome P-450
Fruit development
Fruit set
Fruits
GA4
Gene expression
Genes
Genetic aspects
Hormones
Horticultural crops
Incompatibility
Kinases
Molecular modelling
Ovaries
Parthenocarpy
Parthenogenesis
PbCYP78A6
Pear
Physiological aspects
Plant reproduction
Pollination
Pyrus
RNA-mediated interference
Seeds
Self-incompatibility
Tomatoes
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Summary:Parthenocarpy results in traits attractive to both consumers and breeders, and it overcomes the obstacle of self-incompatibility in the fruit set of horticultural crops, including pear (Pyrus bretshneider). However, there is limited knowledge regarding the genetic and molecular mechanisms that regulate parthenogenesis. Here, in a transcriptional comparison between pollination-dependent fruit and GA.sub.4-induced parthenocarpy, PbCYP78A6 was identified and proposed as a candidate gene involved in parthenocarpy. PbCYP78A6 is similar to Arabidopsis thaliana CYP78A6 and highly expressed in pear hypanthia. The increased PbCYP78A6 expression, as assessed by RT-qPCR, was induced by pollination and GA.sub.4 exposure. The ectopic overexpression of PbCYP78A6 contributed to parthenocarpic fruit production in tomato. The PbCYP78A6 expression coincided with fertilized and parthenocarpic fruitlets development and the expression of fruit development-related genes as assessed by cytological observations and RT-qPCR, respectively. PbCYP78A6 RNA interference and overexpression in pear calli revealed that the gene is an upstream regulator of specific fruit development-related genes in pear. Our findings indicate that PbCYP78A6 plays a critical role in fruit formation and provide insights into controlling parthenocarpy.
ISSN:1471-2229
1471-2229
DOI:10.1186/s12870-021-03098-z