CD44-Targeted Lipid Polymer Hybrid Nanoparticles Enhance Anti-Breast Cancer Effect of Cordyceps militaris Extracts

This study aimed to improve the anticancer effect of herbal extract (CME) on breast cancer cells with hyaluronic acid (HYA) surface-decorated lipid polymer hybrid nanoparticles (LPNPs) and evaluate the applicability of a synthesized poly(glycerol adipate) (PGA) polymer for LPNP preparation. Firstly,...

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Bibliographic Details
Published in:Pharmaceutics 2023-06, Vol.15 (6), p.1771
Main Authors: Suksiriworapong, Jiraphong, Pongprasert, Nutthachai, Bunsupa, Somnuk, Taresco, Vincenzo, Crucitti, Valentina Cuzzucoli, Janurai, Thitapa, Phruttiwanichakun, Pornpoj, Sakchaisri, Krisada, Wongrakpanich, Amaraporn
Format: Article
Language:English
Subjects:
anticancer
Breast cancer
Carotenoids
Cordyceps militaris
Glucose
Glycerol
hyaluronic acid
Ligands
lipid polymer hybrid nanoparticles
Lipids
Nanoparticles
Polyethylene glycol
Polymers
Polyvinyl alcohol
targeted nanoparticles
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Summary:This study aimed to improve the anticancer effect of herbal extract (CME) on breast cancer cells with hyaluronic acid (HYA) surface-decorated lipid polymer hybrid nanoparticles (LPNPs) and evaluate the applicability of a synthesized poly(glycerol adipate) (PGA) polymer for LPNP preparation. Firstly, cholesterol- and vitamin E-grafted PGA polymers (PGA-CH and PGA-VE, respectively) were fabricated, with and without maleimide-ended polyethylene glycol. Subsequently, CME, which contained an active cordycepin equaling 9.89% of its weight, was encapsulated in the LPNPs. The results revealed that the synthesized polymers could be used to prepare CME-loaded LPNPs. The LPNP formulations containing Mal-PEG were decorated with cysteine-grafted HYA via thiol-maleimide reactions. The HYA-decorated PGA-based LPNPs substantially enhanced the anticancer effect of CME against MDA-MB-231 and MCF-7 breast cancer cells by enhancing cellular uptake through CD44 receptor-mediated endocytosis. This study demonstrated the successful targeted delivery of CME to the CD44 receptors of tumor cells by HYA-conjugated PGA-based LPNPs and the new application of synthesized PGA-CH- and PGA-VE-based polymers in LPNP preparation. The developed LPNPs showed promising potential for the targeted delivery of herbal extracts for cancer treatment and clear potential for translation in in vivo experiments.
ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics15061771