Chicken optineurin suppresses MDA5-mediated interferon β production

• Published in: Poultry science 2021-01, Vol.100 (1), p.9-18
• Main Authors: Li, Yu, Jiang, Ning, Mao, Yaqing, Zhang, Wenbo, Xiao, Jing, Wu, Xiangdong, Wu, Huansheng
• Format: Article
• Language: English
• Subjects: Animals; Birnaviridae Infections - immunology; Birnaviridae Infections - veterinary; Cell Cycle Proteins - metabolism; Cell Line; Chick Embryo; chicken interferon β; chicken MDA5; chicken optineurin; Chickens - genetics; Chickens - immunology; Gene Expression Regulation - genetics; Gene Expression Regulation - immunology; Immunology, Health and Disease; Infectious bursal disease virus - physiology; inhibition; Interferon-beta - genetics; Interferon-Induced Helicase, IFIH1 - immunology; RNA, Double-Stranded - genetics
• ISSN: 0032-5791; 1525-3171
• DOI: 10.1016/j.psj.2020.08.020

Chicken MDA5 (chMDA5), the essential accepted pattern recognition receptors for detecting cytoplasmic viral RNA in chicken, initiates interferon β (IFN-β) generation. However, there is an incomplete elucidation of regulating chMDA5-mediated IFN-β production. NEMO-related protein, optineurin, was identified as inhibitors of virus triggered IFN-β induction in human or mice. In this study, full length of chicken optineurin (chOPTN) was cloned from chicken embryo fibroblast, and its role in inhibiting IFN-β signaling pathway was further explored. Full-length chOPTN encodes 547 amino acids residues and contains unique LC3 interaction region and ubiquitin binding domain. Chicken optineurin mRNA and protein are widely expressed in different tissues, especially the heart, kidney, and bursal fabricius (BF). Overexpressed chOPTN not only inhibits poly I:C or homos-induced human IFN-β promoter activation in 293T cells but also suppresses poly I:C, infectious bursal disease virus (IBDV) genome double-strand RNA (dsRNA), and chMDA5-induced chicken IFN-β (chIFN-β) promoter activation. In addition, we first revealed that chOPTN negatively regulates chIFN-β production via inhibiting ubiquitination of chicken TBK1, which is dependent on the ubiquitin-binding domain of chOPTN. Moreover, chIFN-β stimulus, poly I:C, and IBDV genome dsRNA improve chOPTN expression. Endogenous chOPTN expression is also upregulated by IBDV infection in 293T, DF-1 cells, as well as in BF. Therefore, our results suggested that chOPTN plays an inhibition role of chMDA5-mediated chIFN-β signaling pathway in chicken cells.