Ultrastructural Analysis of Prune Dwarf Virus Intercellular Transport and Pathogenesis

(PDV) is an important viral pathogen of plum, sweet cherry, peach, and many herbaceous test plants. Although PDV has been intensively investigated, mainly in the context of phylogenetic relationship of its genes and proteins, many gaps exist in our knowledge about the mechanism of intercellular tran...

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Bibliographic Details
Published in:International journal of molecular sciences 2018-09, Vol.19 (9), p.2570
Main Authors: Kozieł, Edmund, Otulak-Kozieł, Katarzyna, Bujarski, Józef J
Format: Article
Language:English
Subjects:
3D protein structure
Alfalfa
Alfalfa mosaic virus - genetics
Alfalfa mosaic virus - pathogenicity
Alfalfa mosaic virus - ultrastructure
Biological Transport - genetics
Biological Transport - physiology
Cell membranes
Cellular structure
Chloroplasts
Coat protein
Cucumbers
Endoplasmic reticulum
Genomes
immunolocalization
Infections
Leaves
Localization
Mitochondria
Morphology
Movement protein
Organelles
Phylogeny
Plasmodesmata
Proteins
Prune dwarf virus
RNA polymerase
RNA, Viral
Transport
Viral infections
viral intercellular transport
Viral Proteins - metabolism
Viral Proteins - ultrastructure
Virology
Viruses
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Summary:(PDV) is an important viral pathogen of plum, sweet cherry, peach, and many herbaceous test plants. Although PDV has been intensively investigated, mainly in the context of phylogenetic relationship of its genes and proteins, many gaps exist in our knowledge about the mechanism of intercellular transport of this virus. The aim of this work was to investigate alterations in cellular organelles and the cell-to-cell transport of PDV in cv. Polan at ultrastructural level. To analyze the role of viral proteins in local transport, double-immunogold assays were applied to localize PDV coat protein (CP) and movement protein (MP). We observe structural changes in chloroplasts, mitochondria, and cellular membranes. We prove that PDV is transported as viral particles via MP-generated tubular structures through plasmodesmata. Moreover, the computer-run 3D modeling reveals structural resemblances between MPs of PDV and of (AMV), implying similarities of transport mechanisms for both viruses.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms19092570