Genetic Engineering of Crypthecodinium cohnii to Increase Growth and Lipid Accumulation

In this study, we evaluated suitable selected markers and optimized transformation protocols to develop a new genetic transformation methodology for DHA-producing . Additionally, ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), potentially involved in CO fixation under autotrophic conditio...

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Bibliographic Details
Published in:Frontiers in microbiology 2018-03, Vol.9, p.492-492
Main Authors: Diao, Jinjin, Song, Xinyu, Zhang, Xiaoqing, Chen, Lei, Zhang, Weiwen
Format: Article
Language:English
Subjects:
C. cohnii
DHA
genetic modification
metabolomic analysis
Microbiology
RuBisCO
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Summary:In this study, we evaluated suitable selected markers and optimized transformation protocols to develop a new genetic transformation methodology for DHA-producing . Additionally, ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), potentially involved in CO fixation under autotrophic conditions, was selected as the target for construction of a gene knockdown mutant. Our results show that the constructs were successfully inserted into the chromosome by homologous recombination. Comparative analysis showed that deletion of the RuBisCO gene promoted cell growth and increased the lipid content of under heterotrophic conditions compared with those of the wild-type. The liquid chromatography-mass spectrometry (LC-MS) based metabolomic analysis showed that the metabolites involved in energy metabolism were upregulated, suggesting that the deletion of the RuBisCO gene may contribute to the re-direction of more carbon or energy toward growth and lipid accumulation under heterotrophic conditions.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2018.00492