Nanovectorized Microalgal Extracts to Fight Candida albicans and Cutibacterium acnes Biofilms: Impact of Dual-Species Conditions

Biofilm-related infections are a matter of concern especially because of the poor susceptibility of microorganisms to conventional antimicrobial agents. Innovative approaches are needed. The antibiofilm activity of extracts of cyanobacteria , rich in free fatty acids, as well as of extract-loaded co...

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Bibliographic Details
Published in:Antibiotics (Basel) 2020-05, Vol.9 (6), p.279
Main Authors: Lemoine, Virginie, Bernard, Clément, Leman-Loubière, Charlotte, Clément-Larosière, Barbara, Girardot, Marion, Boudesocque-Delaye, Leslie, Munnier, Emilie, Imbert, Christine
Format: Article
Language:English
Subjects:
Acne
Algae
Alginates
Alginic acid
antibiofilm
antimicrobial agent
Antimicrobial agents
bacteria
Bioengineering
Biofilms
Biomaterials
Candida
Candida albicans
Cutibacterium acnes
Cyanobacteria
Fatty acids
Flow cytometry
fungi
Galenic pharmacology
Gram-positive bacteria
Health aspects
Infections
Life Sciences
Lipids
microalga
Microorganisms
Pharmaceutical sciences
polymicrobial biofilm
Species
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Summary:Biofilm-related infections are a matter of concern especially because of the poor susceptibility of microorganisms to conventional antimicrobial agents. Innovative approaches are needed. The antibiofilm activity of extracts of cyanobacteria , rich in free fatty acids, as well as of extract-loaded copper alginate-based nanocarriers, were studied on single- and dual-species biofilms of and . Their ability to inhibit the biofilm formation and to eradicate 24 h old biofilms was investigated. Concentrations of each species were evaluated using flow cytometry. Extracts prevented the growth of single-species biofilms (inhibition > 75% at 0.2 mg/mL) but failed to inhibit preformed biofilms. Nanovectorised extracts reduced the growth of single-species biofilms (inhibition > 43% at 0.2 mg/mL) while free extracts were weakly or not active. Nanovectorised extracts also inhibited preformed biofilms by 55% to 77%, whereas the corresponding free extracts were not active. In conclusion, even if the studied nanocarrier systems displayed promising activity, especially against , their efficacy against dual-species biofilms was limited. This study highlighted that working in such polymicrobial conditions can give a more objective view of the relevance of antibiofilm strategies by taking into account interspecies interactions that can offer additional protection to microbes.
ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics9060279