RPG interacts with E3-ligase CERBERUS to mediate rhizobial infection in Lotus japonicus

• Published in: PLoS genetics 2023-02, Vol.19 (2), p.e1010621
• Main Authors: Li, Xiaolin, Liu, Miaoxia, Cai, Min, Chiasson, David, Groth, Martin, Heckmann, Anne B, Wang, Trevor L, Parniske, Martin, Downie, J Allan, Xie, Fang
• Format: Article
• Language: English
• Subjects: Bacterial infections; Biology and Life Sciences; Cerberus protein; Cytoskeleton; Diseases and pests; Endosomes; Environmental aspects; Exocytosis; Gene Expression Regulation, Plant; Genes; Genotype & phenotype; Infections; Inoculation; Kinases; Legumes; Ligases; Localization; Lotus (Legumes); Lotus - genetics; Lotus - metabolism; Lotus japonicus; Mutation; Nitrogen; Nodulation; Physiological aspects; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots; Proteins; Protoplasts; Research and Analysis Methods; Rhizobium; Rhizobium - genetics; Root hairs; Root nodules; Root Nodules, Plant - genetics; Symbiosis - genetics; Ubiquitin-protein ligase; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism
• ISSN: 1553-7404; 1553-7390; 1553-7404
• DOI: 10.1371/journal.pgen.1010621

Symbiotic interactions between rhizobia and legumes result in the formation of root nodules, which fix nitrogen that can be used for plant growth. Rhizobia usually invade legume roots through a plant-made tunnel-like structure called an infection thread (IT). RPG (Rhizobium-directed polar growth) encodes a coiled-coil protein that has been identified in Medicago truncatula as required for root nodule infection, but the function of RPG remains poorly understood. In this study, we identified and characterized RPG in Lotus japonicus and determined that it is required for IT formation. RPG was induced by Mesorhizobium loti or purified Nodulation factor and displayed an infection-specific expression pattern. Nodule inception (NIN) bound to the RPG promoter and induced its expression. We showed that RPG displayed punctate subcellular localization in L. japonicus root protoplasts and in root hairs infected by M. loti. The N-terminal predicted C2 lipid-binding domain of RPG was not required for this subcellular localization or for function. CERBERUS, a U-box E3 ligase which is also required for rhizobial infection, was found to be localized similarly in puncta. RPG co-localized and directly interacted with CERBERUS in the early endosome (TGN/EE) compartment and near the nuclei in root hairs after rhizobial inoculation. Our study sheds light on an RPG-CERBERUS protein complex that is involved in an exocytotic pathway mediating IT elongation.