Deletion of a Rare Fungal PKS CgPKS11 Promotes Chaetoglobosin A Biosynthesis, Yet Defers the Growth and Development of Chaetomium globosum

We previously reported that chaetoglobosin A (ChA) exhibits a great potential in the biocontrol of nematodes and pathogenic fungi. To improve the production of ChA, a CRISPR-Cas9 system was created and applied for eliminating potential competitive polyketide products. One of the polyketide synthase...

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Published in:Journal of fungi (Basel) 2021-09, Vol.7 (9), p.750
Main Authors: Xiang, Biyun, Hao, Xiaoran, Xie, Qiaohong, Shen, Guangya, Liu, Yanjie, Zhu, Xudong
Format: Article
Language:English
Subjects:
Bacteria
Biological control
Biosynthesis
chaetoglobosin A
chaetoglocin A
Chaetomium globosum
Cloning
CRISPR
Enzymes
Fungi
Gene deletion
Genes
Genomes
Growth rate
Melanin
Metabolites
Peptides
Phylogenetics
Plasmids
Polyketide synthase
polyketide synthases
Proteins
secondary metabolites
sporulation
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Summary:We previously reported that chaetoglobosin A (ChA) exhibits a great potential in the biocontrol of nematodes and pathogenic fungi. To improve the production of ChA, a CRISPR-Cas9 system was created and applied for eliminating potential competitive polyketide products. One of the polyketide synthase encoding genes, Cgpks11, which is putatively involved in the biosynthesis of chaetoglocin A, was disrupted. Cgpks11 deletion led to the overexpression of the CgcheA gene cluster, which is responsible for ChA biosynthesis, and a 1.6-fold increase of ChA. Transcription of pks-1, a melanin PKS, was simultaneously upregulated. Conversely, the transcription of genes for chaetoglocin A biosynthesis, e.g., CHGG_10646 and CHGG_10649, were significantly downregulated. The deletion also led to growth retardation and seriously impaired ascospore development. This study found a novel regulatory means on the biosynthesis of ChA by CgPKS11. CgPKS11 affects chaetoglobosin A biosynthesis, growth, and development in Chaetomium globosum.
ISSN:2309-608X
2309-608X
DOI:10.3390/jof7090750