Separation of individual sulfated bile acid conjugates as calcium complexes using reversed-phase partition thin-layer chromatography

A method for separating individual monosulfated primary bile acid conjugates by reversed-phase partition thin-layer chromatography on octadecyl-bonded silica gel is described. The solvent system is acetonitrile containing calcium, probably as calcium carbamate. Excellent resolution of the 3- and 7-m...

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Bibliographic Details
Published in:Journal of lipid research 1979-08, Vol.20 (6), p.796-800
Main Authors: Raedsch, R, Hofmann, A F, Tserng, K Y
Format: Article
Language:English
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Summary:A method for separating individual monosulfated primary bile acid conjugates by reversed-phase partition thin-layer chromatography on octadecyl-bonded silica gel is described. The solvent system is acetonitrile containing calcium, probably as calcium carbamate. Excellent resolution of the 3- and 7-monosulfated glycine conjugates, as well as 3- and 7-monosulfated taurine conjugates of cholic and chenodeoxycholic acids is reported. A convenient class separation of sulfated from nonsulfated primary bile acid conjugates by adsorption thin-layer chromatography on low-polarity silica gel is also described.
ISSN:0022-2275
1539-7262
DOI:10.1016/S0022-2275(20)40033-1