Impact of un-polymerized acrylamide monomer residues onto protein identification by MALDI TOF MS

Polyacrylamide gel electrophoresis is a powerful tool for protein mixture separation frequently used in proteomic studies. This article describes the problems which can arise during the identification process of separated proteins by matrix assisted laser desorption/ionization mass spectrometry. Pre...

Full description

Saved in:
Bibliographic Details
Published in:Central European journal of chemistry 2012-08, Vol.10 (4), p.1073-1078
Main Authors: Talian, Ivan, Kováčová, Veronika, Petrovič, Marián, Sabo, Ján
Format: Article
Language:English
Subjects:
Acrylamide
Biochemistry
Biological and Medical Physics
Biophysics
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Gel electrophoresis
Geochemistry
MALDI
Peptide mass fingerprinting
Research Article
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Polyacrylamide gel electrophoresis is a powerful tool for protein mixture separation frequently used in proteomic studies. This article describes the problems which can arise during the identification process of separated proteins by matrix assisted laser desorption/ionization mass spectrometry. Presence of residual acrylamide monomers in the peptide samples leads to a significant peptide signal suppression. In the case of protein prohibitin, isolated from the MCF 7 breast cancer cell line, the peptide signal suppression was observed in 50% of all detected peptides.
ISSN:1895-1066
2391-5420
1644-3624
2391-5420
DOI:10.2478/s11532-012-0024-3