Characteristics of Interstitial Fibrosis and Inflammatory Cell Infiltration in Right Ventricles of Systemic Sclerosis-Associated Pulmonary Arterial Hypertension

Systemic sclerosis-associated pulmonary arterial hypertension (SScPAH) has a disturbed function of the right ventricle (RV) when compared to idiopathic PAH (IPAH). Systemic sclerosis may also affect the heart. We hypothesize that RV differences may occur at the level of interstitial inflammation and...

Full description

Saved in:
Bibliographic Details
Published in:International Journal of Rheumatology 2010-01, Vol.2010 (2010), p.99-108
Main Authors: Dijkmans, Ben C., Vonk-Noordegraaf, Anton, Grünberg, Katrien, Belien, Jeroen A. M., Voskuyl, Alexandre E., Boonstra, Anco, Kupreishvili, Koba, Hadi, Awal M., Niessen, Hans M., Mouchaers, Koen T. B., Overbeek, Maria J., Smit, Egbert F.
Format: Article
Language:English
Subjects:
Cardiomyocytes
Conflicts of interest
Cytokines
Disease
Family medical history
Inflammation
Ischemia
Mortality
Patients
Pulmonary arteries
Pulmonary hypertension
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Systemic sclerosis-associated pulmonary arterial hypertension (SScPAH) has a disturbed function of the right ventricle (RV) when compared to idiopathic PAH (IPAH). Systemic sclerosis may also affect the heart. We hypothesize that RV differences may occur at the level of interstitial inflammation and—fibrosis and compared inflammatory cell infiltrate and fibrosis between the RV of SScPAH, IPAH, and healthy controls. Methods. Paraffin-embedded tissue samples of RV and left ventricle (LV) from SScPAH (n=5) and IPAH (n=9) patients and controls (n=4) were picrosirius red stained for detection of interstitial fibrosis, which was quantified semiautomatically. Neutrophilic granulocytes (MPO), macrophages (CD68), and lymphocytes (CD45) were immunohistochemically stained and only interstitial leukocytes were counted. Presence of epi- or endocardial inflammation, and of perivascular or intimal fibrosis of coronary arteries was assessed semiquantitatively (0–3: absent to extensive). Results. RV's of SScPAH showed significantly more inflammatory cells than of IPAH (cells/mm2, mean±sd MPO 11±3 versus 6±1; CD68 11±3 versus 6±1; CD45 11±1 versus 5±1 , P
ISSN:1687-9260
1687-9279
DOI:10.1155/2010/604615