Phenotypic and molecular characterization of Salmonella Enteritidis strains isolated in São Paulo, Brazil

In São Paulo State, Brazil, the epidemic increase in isolation of Salmonella Enteritidis has been observed since 1994. A total of 105 S. Enteritidis strains (72 from human and 33 from non-human sources) isolated during the period 1975-1995, previously characterized by phage typing, was analyzed by a...

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Published in:Revista do Instituto de Medicina Tropical de São Paulo 2003-04, Vol.45 (2), p.59-63
Main Authors: Fernandes, Sueli A, Ghilardi, Angela C R, Tavechio, Ana T, Machado, Antonia M O, Pignatari, Antonio C C
Format: Article
Language:English
Subjects:
Antimicrobial resistance
Bacteriophage Typing
Brazil
DNA, Bacterial - analysis
Drug Resistance, Bacterial
Humans
Microbial Sensitivity Tests
Phagetypes
Phenotype
Plasmids
Plasmids - classification
Plasmids - genetics
Ribotypes
Ribotyping
Salmonella Enteritidis
Salmonella enteritidis - classification
Salmonella enteritidis - drug effects
Salmonella enteritidis - genetics
TROPICAL MEDICINE
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Summary:In São Paulo State, Brazil, the epidemic increase in isolation of Salmonella Enteritidis has been observed since 1994. A total of 105 S. Enteritidis strains (72 from human and 33 from non-human sources) isolated during the period 1975-1995, previously characterized by phage typing, was analyzed by antimicrobial susceptibility, plasmid profile, and ribotyping. Over 70% of the strains were susceptible to all antimicrobial agents tested, however, multiple resistance to antimicrobials was observed among the studied strains, mainly those from hospitalized patients. Phage type 8 (PT-8) was predominant among the strains isolated during the period of 1975-1992, but in the following years, PT-4 was the most frequent phage type identified. Seven different plasmid profiles were detected and 96% of the isolates harbored a plasmid of approximately 36 MDa. Ribotyping discriminated fourteen ribotypes (R1 to R14) among the strains examined. By analysis of dendrogram the strains were included in three groups with similarity level of 60%. The obtained results indicate that, a single ribotype (R11), determined for PT-4 strains isolated from 1993, characterizes the epidemic clone of S. Enteritidis in our region.
ISSN:0036-4665
1678-9946
0036-4665
1678-9946
DOI:10.1590/S0036-46652003000200001