The CB1 cannabinoid receptor regulates autophagy in the tibialis anterior skeletal muscle in mice

The endocannabinoid system (ECS) regulates energy metabolism, has been implicated in the pathogenesis of metabolic diseases and exerts its actions mainly through the type 1 cannabinoid receptor (CB1). Likewise, autophagy is involved in several cellular processes. It is required for the normal develo...

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Bibliographic Details
Published in:Biological research 2023-03, Vol.56 (1), p.14-14, Article 14
Main Authors: Sepúlveda, Carlos, Rodríguez, Juan Manuel, Monsalves-Álvarez, Matías, Donoso-Barraza, Camila, Pino-de la Fuente, Francisco, Matías, Isabelle, Leste-Lasserre, Thierry, Zizzari, Philippe, Morselli, Eugenia, Cota, Daniela, Llanos, Miguel, Troncoso, Rodrigo
Format: Article
Language:English
Subjects:
Animals
Autophagy
Autophagy - physiology
BIOLOGY
Cannabinoid CB1 receptors
Cannabinoids
Cannabinoids - metabolism
Diabetes
Endocannabinoid receptor
Endocannabinoid system
Energy metabolism
Enzymes
Fatty acids
Genotype & phenotype
High fat diet
Life Sciences
Metabolic disorders
Mice
Mice, Inbred C57BL
Mice, Obese
Muscle, Skeletal - metabolism
Muscles
Musculoskeletal system
Nervous system
Neurons and Cognition
Obesity
Phosphorylation
Physiological aspects
Proteins
Receptor, Cannabinoid, CB1 - metabolism
Skeletal muscle
TOR protein
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Summary:The endocannabinoid system (ECS) regulates energy metabolism, has been implicated in the pathogenesis of metabolic diseases and exerts its actions mainly through the type 1 cannabinoid receptor (CB1). Likewise, autophagy is involved in several cellular processes. It is required for the normal development of muscle mass and metabolism, and its deregulation is associated with diseases. It is known that the CB1 regulates signaling pathways that control autophagy, however, it is currently unknown whether the ECS could regulate autophagy in the skeletal muscle of obese mice. This study aimed to investigate the role of the CB1 in regulating autophagy in skeletal muscle. We found concomitant deregulation in the ECS and autophagy markers in high-fat diet-induced obesity. In obese CB1-KO mice, the autophagy-associated protein LC3 II does not accumulate when mTOR and AMPK phosphorylation levels do not change. Acute inhibition of the CB1 with JD-5037 decreased LC3 II protein accumulation and autophagic flux. Our results suggest that the CB1 regulates autophagy in the tibialis anterior skeletal muscle in both lean and obese mice.
ISSN:0717-6287
0716-9760
0717-6287
DOI:10.1186/s40659-023-00426-5